Haplotype combinations of AdPLA gene polymorphisms associate with growth traits in Chinese cattle
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Ablation of AdPLA in mice could prevent obesity, which implicated AdPLA was a new candidate gene for growth traits. In this study, we screened the polymorphisms of AdPLA gene and revealed three novel SNPs (NC_007330.4:g.43638506 C>T, 43658457 T>C and 43661404 T>C) by DNA sequencing and forced PCR-RFLP methods in 1340 individuals from six breeds. A preliminary single SNP-traits association analysis demonstrated that CC genotype was significantly higher than TT genotype at two growth traits in P6-EcoRII locus (P < 0.05). Moreover, the P8-FbaI locus had a significant association with some growth traits, individuals with TT genotype were higher than CC genotype (P < 0.05). A further analysis confirmed that these two SNPs were in linkage disequilibrium, and haplotype H2, both advantage allelic in P6-EcoRII and P8-FbaI loci, was better than the others for bovine growth traits, which was consistent with single SNP-traits association results. Therefore, this study not only proved that these SNPs were in association with bovine growth traits, but also contributed to evaluate them as genetic markers in bovine genetics and breeding and had potential application in breeding programs.
KeywordsAdPLA gene Cattle Forced PCR-RFLP Haplotype SNP
This study was supported by the National Natural Science Foundation of China (Grant No. 30972080), National Key Technology R&D Program (Grant No. 2008ADB2B03-19), Keystone Project of Transfergene in China (Grant No. 2009ZX08009-157B, 2008ZX08007-002, and 2009ZX08007-005B-07), Program of National Beef Cattle Industrial Technology System (CARS-38).
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