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Molecular Biology Reports

, Volume 39, Issue 6, pp 6907–6914 | Cite as

IL-1β (−511T/C) gene polymorphism not IL-1β (+3953T/C) and LT-α (+252A/G) gene variants confers susceptibility to visceral leishmaniasis

  • Ali Moravej
  • Manoochehr Rasouli
  • Mehdi Kalani
  • Sadaf Asaei
  • Simin Kiany
  • Sohrab Najafipour
  • Amin Koohpayeh
  • Abbas Abdollahi
Article

Abstract

Lymphotoxin-α (LT-α) and interleukin-1beta (IL-1β) are proinflammatory cytokines playing important roles in immunity against Leishmania infection and the outcome of the disease. As cytokine productions are under the genetic control, this study tried to find any probable relationship between these cytokine gene polymorphisms and the susceptibility to visceral leishmaniasis in Iranian pediatric patients. Ninety-five pediatric patients involved with visceral leishmaniasis and 128 non-relative healthy people, from the same area as the patients, were genotyped for LT-α (+252A/G) and IL-1β (+3953T/C and −511T/C) gene polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP). There was not found any significant differences in allele and genotype frequencies of LT-α (+252A/G) and IL-1β (+3953) among the study groups. However, the frequency of IL-1β −511TT genotype was higher in the controls (P = 0.0004) while the frequency of IL-1β −511CC genotype and C allele were higher in the patients (P = 0.008 and P = 0.00006, respectively). Furthermore, IL-1β CC (−511/+3953) haplotype was more frequent in VL patients compared with the controls (P = 0.0002) and the distribution of TT haplotype was higher in the controls compared with the patients (P = 0.003). In conclusion, based on the results, IL-1β −511C allele, CC genotype and CC (−511/+3953) haplotype could be considered as the susceptibility factors for visceral leishmaniasis while IL-1β −511TT genotype, T allele and TT haplotype (−511/+3953) might be counted as the influential factors for resistance to the disease.

Keywords

LT-α IL-1β Visceral leishmaniasis Genetic polymorphism 

Notes

Acknowledgment

We would like to thank Miss. Esmat Kazemi for fundamental English editing. This study was financially supported by a Grant No. 85-3 from Prof. Alborzi Clinical Microbiology Research Center affiliated with Shiraz University of Medical Sciences.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ali Moravej
    • 1
  • Manoochehr Rasouli
    • 2
  • Mehdi Kalani
    • 2
  • Sadaf Asaei
    • 2
  • Simin Kiany
    • 2
  • Sohrab Najafipour
    • 1
  • Amin Koohpayeh
    • 1
  • Abbas Abdollahi
    • 1
  1. 1.Department of MicrobiologyFasa University of Medical SciencesFasaIran
  2. 2.Department of Immunology, Prof. Alborzi Clinical Microbiology Research CenterShiraz University of Medical SciencesShirazIran

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