Molecular Biology Reports

, Volume 39, Issue 5, pp 5851–5857

Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing

  • Cai-Hua Dong
  • Chen Li
  • Xiao-Hong Yan
  • Shun-Mou Huang
  • Jin-Yong Huang
  • Li-Jun Wang
  • Rui-Xing Guo
  • Guang-Yuan Lu
  • Xue-Kun Zhang
  • Xiao-Ping Fang
  • Wen-Hui Wei
Article

DOI: 10.1007/s11033-011-1395-9

Cite this article as:
Dong, CH., Li, C., Yan, XH. et al. Mol Biol Rep (2012) 39: 5851. doi:10.1007/s11033-011-1395-9

Abstract

Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and metabolic processes. Our results provide useful information for further analyses of the drought stress tolerance in Sinapis, and will facilitate molecular breeding for Brassica crop plants.

Keywords

Sinapis alba Drought stress Illumina sequencing Gene expression Drought tolerance genes 

Supplementary material

11033_2011_1395_MOESM1_ESM.xls (97 kb)
Supplementary material 1 (XLS 97 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Cai-Hua Dong
    • 1
  • Chen Li
    • 2
  • Xiao-Hong Yan
    • 1
  • Shun-Mou Huang
    • 1
  • Jin-Yong Huang
    • 3
  • Li-Jun Wang
    • 1
  • Rui-Xing Guo
    • 1
  • Guang-Yuan Lu
    • 1
  • Xue-Kun Zhang
    • 1
  • Xiao-Ping Fang
    • 1
  • Wen-Hui Wei
    • 1
  1. 1.Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crop Biology and Genetic Breeding of the Ministry of AgricultureWuhanChina
  2. 2.College of Food Science and TechnologyAgricultural University of HebeiBaodingChina
  3. 3.Department of BioengineeringZhengzhou UniversityZhengzhouChina

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