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Molecular Biology Reports

, Volume 39, Issue 5, pp 5307–5313 | Cite as

Genetic diversity in some grape varieties revealed by SCoT analyses

  • Da-Long Guo
  • Jun-Yu Zhang
  • Chong-Huai Liu
Article

Abstract

Start codon targeted (SCoT) polymorphic markers were used to assess genetic relationships among 64 grape varieties. Seventeen informative primers were selected from 36 SCoT primers based on their ability to produce clear and repeatable polymorphic and unambiguous bands among the varieties. A total of 131 bands were produced; 93.1% of them were polymorphic; the average polymorphism information content was 0.82. Cluster analysis of SCoT markers through the unweighted pair-group method of arithmetic averages analysis and principal coordinate analysis were largely consistent. The partition of clusters in the dendrogram and PCoA plot was similar and some degree of grouping by types of grape and taxonomic status of the varieties was revealed. Four main groups were found after cluster analysis, i.e. table grape of Vitis vinifera; table grape of Euro-America hybrid; wine grape of V. vinifera and wild Vitis species. The results showed that the wild Vitis species originated from America and China could be clearly differentiated. The results also indicated that SCoT markers are informative and could be used to detect polymorphism for grape varieties.

Keywords

Genetic diversity SCoT Grape Vitis 

Notes

Acknowledgments

This study was financially supported by Natural Science Foundation of China (NSFC: 30800742), Young Teacher Funding Program of Henan Higher School (2010GGJS-072) and National Technology System for Grape Industry of China (nycytx-30-zy-01).

Supplementary material

11033_2011_1329_MOESM1_ESM.doc (114 kb)
Supplementary material 1 (DOC 110 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.College of ForestryHenan University of Science and TechnologyLuoyangPeople’s Republic of China
  2. 2.Zhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesZhengzhouPeople’s Republic of China

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