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Molecular Biology Reports

, Volume 39, Issue 4, pp 4831–4835 | Cite as

Influence of the interaction between the adiponectin G276T polymorphism and body mass index on lipid levels in healthy children

  • Pía Riestra
  • Alicia García-Anguita
  • Miguel A. Lasunción
  • Alipio Mangas
  • Manuel de Oya
  • Carmen Garcés
Article

Abstract

Adiponectin is an adipose tissue-specific hormone which is inversely associated with metabolic alterations related to atherosclerosis. Polymorphisms in the adiponectin gene (AdipoQ) have been related to low adiponectin levels as well as several cardiovascular risk factors, but this association remains controversial. In our study we investigated the relationship between the AdipoQ T45G (rs: 2241766) and G276T (rs: 1501299) polymorphisms and adiponectin concentrations, blood pressure, and lipid and insulin levels, in a population-based sample of 12- to 16-year-old children. The study included 815 healthy Spanish children (388 boys and 427 girls). Plasma glucose and lipid levels were determined by standard methods. Insulin concentrations were measured by RIA, and serum adiponectin levels were determined by ELISA. The AdipoQ T45G and AdipoQ G276T polymorphisms were determined by TaqMan® allelic discrimination assays. ANOVA or t test allowed for comparison of the studied parameters across genotypes or genotype groups, respectively. A linear regression analysis was performed to examine the independent relationships of the lipid variables with BMI (body mass index), AdipoQ G276T polymorphism and the interaction between the two. When independently comparing the effect of these polymorphisms in normal-weight and overweight children, we observed that overweight boys carriers of the minor allele T had significantly lower TC, LDL-C and apo A-I levels than non-carriers, but these differences were not apparent in normal-weight boys. Furthermore, linear regression analysis demonstrated that interaction between the BMI and the AdipoQ G276T polymorphism is a significant factor explaining the variations of TC and LDL-C levels. To our knowledge, this is the first study to report an association between the AdipoQ G276T polymorphism and lipid levels in overweight boys alone, thereby suggesting that the influence of the AdipoQ polymorphisms on cardiovascular risk factors may be dependent on BMI.

Keywords

Adiponectin polymorphisms BMI Lipid levels Children 

Notes

Acknowledgment

This study was supported by a grant from the Fondo de Investigación Sanitaria (FIS 08/1189). The contract of Carmen Garcés is co-financed by the Fondo de Investigación Sanitaria. Pía Riestra and Alicia García-Anguita are fellows of the Conchita Rábago Foundation. We thank Oliver Shaw for his revision of our manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Pía Riestra
    • 1
  • Alicia García-Anguita
    • 1
  • Miguel A. Lasunción
    • 2
  • Alipio Mangas
    • 3
  • Manuel de Oya
    • 1
  • Carmen Garcés
    • 1
  1. 1.Lipid Metabolism LaboratoryIIS-Fundación Jiménez DíazMadridSpain
  2. 2.Servicio de Bioquímica-InvestigaciónHospital Universitario Ramón y Cajal, IRYCIS, and CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), ISCIIIMadridSpain
  3. 3.Department of MedicineUniversidad de CádizCadizSpain

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