Molecular Biology Reports

, Volume 39, Issue 4, pp 4797–4802 | Cite as

Effect of surgical castration on expression of TRPM8 in urogenital tract of male rats

  • Zhonghua Yang
  • Xinghuan Wang
  • Guangbin Zhu
  • Zhangyan Zhou
  • Yongzhi Wang
  • Dong Chen
  • Zhe Meng


Trpm8 (melastatin-related transient receptor potential member 8), a member of the transient receptor potential (TRP) superfamily, encoding a cation channel named TRPM8, has been shown to be a primary androgen-responsive gene and play an important role in prostate physiology. To investigate the expression feature of TRPM8 in urogenital tract of male rats, and whether TRPM8 was also regulated by androgen receptor in these organs, male Sprague–Dawley rats were divided into three groups of 35 animals as follows: sham-operated (SHAM), orchidectomized (ORX), orchidectomized plus DHT treatment (O + D). Organs in urogenital tract, including kidney, prostate, seminal vesicle (SV), testis, epididymis and penis, were collected at different post-castration periods. RT-PCR, real-time PCR and Western blotting were used to detect the expression of androgen receptor (AR) and trpm8 in these tissue. As a result, AR and trpm8 can be detected in all these organs at mRNA or/and protein level. The mRNA expression of trpm8 in kidney, prostate, SV and penis decreased 24 or 72 h after castration and kept decreasing in a time-dependant manner. However, treatment of dihydrotestosterone (DHT) could reverse the effect of surgical castration. Collectively, our data provide evidence that TRPM8 and AR were expressed generally in urogenital tract of male rats, and in these organs, expression of trpm8 was regulated by serum androgen.


Androgen Castration Transient receptor potential (TRP) channels Urogenital tract 



This work was supported by grants from NSF China (No. 30872572) and Natural Science Foundation of Guangdong Province of China (No. 7001197).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zhonghua Yang
    • 1
  • Xinghuan Wang
    • 1
  • Guangbin Zhu
    • 1
  • Zhangyan Zhou
    • 1
  • Yongzhi Wang
    • 1
  • Dong Chen
    • 1
  • Zhe Meng
    • 1
  1. 1.Department of UrologyZhongnan Hospital of Wuhan UniversityWuhanChina

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