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Molecular Biology Reports

, Volume 39, Issue 4, pp 4797–4802 | Cite as

Effect of surgical castration on expression of TRPM8 in urogenital tract of male rats

  • Zhonghua Yang
  • Xinghuan Wang
  • Guangbin Zhu
  • Zhangyan Zhou
  • Yongzhi Wang
  • Dong Chen
  • Zhe Meng
Article

Abstract

Trpm8 (melastatin-related transient receptor potential member 8), a member of the transient receptor potential (TRP) superfamily, encoding a cation channel named TRPM8, has been shown to be a primary androgen-responsive gene and play an important role in prostate physiology. To investigate the expression feature of TRPM8 in urogenital tract of male rats, and whether TRPM8 was also regulated by androgen receptor in these organs, male Sprague–Dawley rats were divided into three groups of 35 animals as follows: sham-operated (SHAM), orchidectomized (ORX), orchidectomized plus DHT treatment (O + D). Organs in urogenital tract, including kidney, prostate, seminal vesicle (SV), testis, epididymis and penis, were collected at different post-castration periods. RT-PCR, real-time PCR and Western blotting were used to detect the expression of androgen receptor (AR) and trpm8 in these tissue. As a result, AR and trpm8 can be detected in all these organs at mRNA or/and protein level. The mRNA expression of trpm8 in kidney, prostate, SV and penis decreased 24 or 72 h after castration and kept decreasing in a time-dependant manner. However, treatment of dihydrotestosterone (DHT) could reverse the effect of surgical castration. Collectively, our data provide evidence that TRPM8 and AR were expressed generally in urogenital tract of male rats, and in these organs, expression of trpm8 was regulated by serum androgen.

Keywords

Androgen Castration Transient receptor potential (TRP) channels Urogenital tract 

Notes

Acknowledgments

This work was supported by grants from NSF China (No. 30872572) and Natural Science Foundation of Guangdong Province of China (No. 7001197).

References

  1. 1.
    Berridge MJ, Lipp P, Bootman MD (2000) The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 1(1):11–21. doi: 10.1038/35036035 PubMedCrossRefGoogle Scholar
  2. 2.
    Liu FF, Ma ZY, Li DL, Feng JB, Zhang K, Wang R, Zhang W, Li L, Zhang Y (2010) Differential expression of TRPC channels in the left ventricle of spontaneously hypertensive rats. Mol Biol Rep 37(6):2645–2651. doi: 10.1007/s11033-009-9792-z PubMedCrossRefGoogle Scholar
  3. 3.
    Yuan FL, Chen FH, Lu WG, Li X (2010) Acid-sensing ion channels 3: a potential therapeutic target for pain treatment in arthritis. Mol Biol Rep 37(7):3233–3238. doi: 10.1007/s11033-009-9907-6 PubMedCrossRefGoogle Scholar
  4. 4.
    Tsavaler L, Shapero MH, Morkowski S, Laus R (2001) Trp-p8, a novel prostate-specific gene, is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins. Cancer Res 61(9):3760–3769PubMedGoogle Scholar
  5. 5.
    Yang ZH, Wang XH, Wang HP, Hu LQ, Zheng XM, Li SW (2010) Capsaicin mediates cell death in bladder cancer T24 cells through reactive oxygen species production and mitochondrial depolarization. Urology 75(3):735–741. doi: 10.1016/j.urology.2009.03.042 PubMedCrossRefGoogle Scholar
  6. 6.
    Zhang L, Jones S, Brody K, Costa M, Brookes SJ (2004) Thermosensitive transient receptor potential channels in vagal afferent neurons of the mouse. Am J Physiol Gastrointest Liver Physiol 286(6):G983–G991. doi: 10.1152/ajpgi.00441.2003 PubMedCrossRefGoogle Scholar
  7. 7.
    Mustafa S, Oriowo M (2005) Cooling-induced contraction of the rat gastric fundus: mediation via transient receptor potential (TRP) cation channel TRPM8 receptor and Rho-kinase activation. Clin Exp Pharmacol Physiol 32(10):832–838. doi: 10.1111/j.1440-1681.2005.04273.x PubMedCrossRefGoogle Scholar
  8. 8.
    Yang XR, Lin MJ, McIntosh LS, Sham JS (2006) Functional expression of transient receptor potential melastatin- and vanilloid-related channels in pulmonary arterial and aortic smooth muscle. Am J Physiol Lung Cell Mol Physiol 290(6):L1267–L1276. doi: 10.1152/ajplung.00515.2005 PubMedCrossRefGoogle Scholar
  9. 9.
    Henshall SM, Afar DE, Hiller J, Horvath LG, Quinn DI, Rasiah KK, Gish K, Willhite D, Kench JG, Gardiner-Garden M, Stricker PD, Scher HI, Grygiel JJ, Agus DB, Mack DH, Sutherland RL (2003) Survival analysis of genome-wide gene expression profiles of prostate cancers identifies new prognostic targets of disease relapse. Cancer Res 63(14):4196–4203PubMedGoogle Scholar
  10. 10.
    Stein RJ, Santos S, Nagatomi J, Hayashi Y, Minnery BS, Xavier M, Patel AS, Nelson JB, Futrell WJ, Yoshimura N, Chancellor MB, De Miguel F (2004) Cool (TRPM8) and hot (TRPV1) receptors in the bladder and male genital tract. J Urol 172(3):1175–1178. doi: 10.1097/01.ju.0000134880.55119.cf PubMedCrossRefGoogle Scholar
  11. 11.
    Zhang L, Barritt GJ (2004) Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells. Cancer Res 64(22):8365–8373PubMedCrossRefGoogle Scholar
  12. 12.
    Bidaux G, Roudbaraki M, Merle C, Crepin A, Delcourt P, Slomianny C, Thebault S, Bonnal JL, Benahmed M, Cabon F, Mauroy B, Prevarskaya N (2005) Evidence for specific TRPM8 expression in human prostate secretory epithelial cells: functional androgen receptor requirement. Endocr Relat Cancer 12(2):367–382PubMedCrossRefGoogle Scholar
  13. 13.
    Eggener SE, Stern JA, Jain PM, Oram S, Ai J, Cai X, Roehl KA, Wang Z (2006) Enhancement of intermittent androgen ablation by “off-cycle” maintenance with finasteride in LNCaP prostate cancer xenograft model. Prostate 66(5):495–502. doi: 10.1002/pros.20297 PubMedCrossRefGoogle Scholar
  14. 14.
    Wang Y, Gupta S, Hua V, Ramos-Garcia R, Shevrin D, Jovanovic BD, Nelson JB, Wang Z (2010) Prolongation of off-cycle interval by finasteride is not associated with survival improvement in intermittent androgen deprivation therapy in LNCaP tumor model. Prostate 70(2):147–154. doi: 10.1002/pros.21046 PubMedGoogle Scholar
  15. 15.
    Vanden Abeele F, Skryma R, Shuba Y, Van Coppenolle F, Slomianny C, Roudbaraki M, Mauroy B, Wuytack F, Prevarskaya N (2002) Bcl-2-dependent modulation of Ca(2+) homeostasis and store-operated channels in prostate cancer cells. Cancer Cell 1(2):169–179CrossRefGoogle Scholar
  16. 16.
    Sar M, Lubahn DB, French FS, Wilson EM (1990) Immunohistochemical localization of the androgen receptor in rat and human tissues. Endocrinology 127(6):3180–3186PubMedCrossRefGoogle Scholar
  17. 17.
    Prins GS, Birch L (1993) Immunocytochemical analysis of androgen receptor along the ducts of the separate rat prostate lobes after androgen withdrawal and replacement. Endocrinology 132(1):169–178PubMedCrossRefGoogle Scholar
  18. 18.
    Paris F, Weinbauer GF, Blum V, Nieschlag E (1994) The effect of androgens and antiandrogens on the immunohistochemical localization of the androgen receptor in accessory reproductive organs of male rats. J Steroid Biochem Mol Biol 48(1):129–137PubMedCrossRefGoogle Scholar
  19. 19.
    Blondeau JP, Baulieu EE, Robel P (1982) Androgen-dependent regulation of androgen nuclear receptor in the rat ventral prostate. Endocrinology 110(6):1926–1932PubMedCrossRefGoogle Scholar
  20. 20.
    Kyprianou N, Isaacs JT (1988) Activation of programmed cell death in the rat ventral prostate after castration. Endocrinology 122(2):552–562PubMedCrossRefGoogle Scholar
  21. 21.
    Schoenmakers E, Verrijdt G, Peeters B, Verhoeven G, Rombauts W, Claessens F (2000) Differences in DNA binding characteristics of the androgen and glucocorticoid receptors can determine hormone-specific responses. J Biol Chem 275(16):12290–12297PubMedCrossRefGoogle Scholar
  22. 22.
    Nantermet PV, Xu J, Yu Y, Hodor P, Holder D, Adamski S, Gentile MA, Kimmel DB, Harada S, Gerhold D, Freedman LP, Ray WJ (2004) Identification of genetic pathways activated by the androgen receptor during the induction of proliferation in the ventral prostate gland. J Biol Chem 279(2):1310–1322. doi: 10.1074/jbc.M310206200 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zhonghua Yang
    • 1
  • Xinghuan Wang
    • 1
  • Guangbin Zhu
    • 1
  • Zhangyan Zhou
    • 1
  • Yongzhi Wang
    • 1
  • Dong Chen
    • 1
  • Zhe Meng
    • 1
  1. 1.Department of UrologyZhongnan Hospital of Wuhan UniversityWuhanChina

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