Gene expression profiles of two intraspecific Larix lines and their reciprocal hybrids
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Heterosis has been widely explored in Larix breeding for more than a century, but the molecular mechanisms underlying this phenomenon remain elusive. In the present study, the genome-wide transcript profiles from two Larix genotypes and their reciprocal hybrids were analyzed using Arabidopsis 70-mer oligonucleotide microarrays. Despite sharing the same two parental lines, one of the hybrids showed obvious heterosis, while the other did not. In total, 1,171 genes were differentially expressed between the heterotic hybrid and its parents, of which 133 genes were nonadditive expression. The number of differentially expressed genes between the non-heterotic hybrid and the parents was 939, but only 54 of these genes were nonadditive expression. Further, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analyses indicated that most of these differentially expressed genes in the heterotic hybrid were associated with several important biological functions such as physiological processes, responses to stimulus, and starch and sucrose metabolism. The reliability of the microarray data was further validated by the Real-time quantitative RT-PCR. A high Pearson linear correlation coefficient value was detected (r = 0.759, P < 0.01). In conclusion, the gene expression profile in the Larix heterotic hybrid was significantly different from that obtained from the non-heterotic hybrid, and more nonadditive differentially expressed genes were detected in the heterotic hybrid, implying that nonadditive effects may be closely associated with the formation of heterosis in the intraspecific Larix hybridization.
KeywordsLarix kaempferi (Lamb.) Heterosis Nonadditive expression Transcript profile
Firstly, we thank the anonymous reviewers for critical reading of the manuscript. We are also grateful to Dr. Liwang Qi, Chinese Academy of Forestry, Beijing, China, for kindly providing the materials of Larix kaempferi (Lamb.). This work was carried out with the financial support from The National Key Basic Research Program (N0. 2009CB119105) and The National Natural Science Foundation of China and Tianjin (N0. 10JCZDJC17900 and N0. 07JCYBJC11700).
- 3.Duvick DN (1997) Heterosis: feeding people and protecting natural resources. In: Coors JG, Pandey S (eds) Genetics and exploitation of heterosis in crops. American Society of Agronomy, Madison, pp 19–29Google Scholar
- 7.Fisher RA (1918) The correlation between relatives on the supposition of Mendelian inheritance. Trans Roy soc 52:399–433Google Scholar
- 13.Chen NH, Gao YP, Yang JS, Qian M, Ge KL (1997) Alteration of gene expression in rice hybrid F1 and its parental seedlings. Acta Bot Sin 39:379–382Google Scholar
- 21.Fujimoto T, Akutsu H, Kita K, Uchiyama K, Kuromaru M (2004) Variation in the density in hybrid larch (Larix gmelinii var. japonica × L. kaempferi) F1. Abstracts of the 54th Annual Meeting of Japan Wood Research Society (in Japanese), pp 519Google Scholar
- 22.Takahashi N, Nishiguchi C (1966) Studies on the resistance of forest trees to the red-backed vole, Clethrionomys rufocanus bedfordiae (Thomas). (2) Relative feeding preference of the vole for seedings of larch F1 hybrids under laboratory conditions. Bull Tokyo Univ Forest 62:173–188 (in Japanese with English summary)Google Scholar
- 23.Fukuchi M (1987) A comparison of Kurile larch × Japanese larch with Japanese larch in vole-resistance in a mixed stand. Transactions of the 98th Annual Meeting of the Japanese Forest society (in Japanese), pp 257–258Google Scholar
- 24.Oshima T, Hatakeyama S, Kubota Y (1974) Characteristics of generations of hybrid larch. I. Larix gmelinii var japonica × L. kaempferi hybrid larch F1 and their double cross species. Transactions of the 85th Annual Meeting of the Japanese Forest society (in Japanese), pp 169–171Google Scholar
- 25.Kaji K, Hatakeyama S, Fujitani M (1974) Characteristics of generations of hybrid larch. II. Larix gmelinii var japonica × L. kaempferi hybrid larch F1 and their back cross species. Transactions of the 85th Annual Meeting of the Japanese Forest society (in Japanese), pp 172–173Google Scholar
- 35.Zhuang J, Chen JM, Yao QH, Xiong F, Sun CC, Zhou XR, Zhang J, Xiong AS (2010) Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Mol Biol Rep. doi: 10.1007/s11033-010-0162-7