A comparative search for human FcεRIα gene (FCER1A) 3′-UTR polymorphisms in Japanese and Polish populations
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The high affinity immunoglobulin E (IgE) receptor (FcεRI) plays a key role in the pathogenesis of atopy and allergic disorders. Several polymorphisms located in 5′-flanking region and 5′-untranslated region (5′-UTR) of human FCER1A, the gene encoding FcεRI α-subunit, have been shown to functionally affect its transcriptional activity. All those genetic variants have been also associated with allergic diseases and/or serum IgE levels. In the present study, we sought to identify functional polymorphisms in human FCER1A 3′-untranslated region (3′-UTR), the potential candidates for future genetic association studies. Search for polymorphisms within human FCER1A 3′-UTR region, conducted in Japanese and Poles, revealed the presence of +5650A>G and +5714G>A variants. Subsequently, structure/distribution of haplotypes and LD measures were analyzed in Japanese and Poles for both 3′-UTR variants and the functional polymorphisms located in 5′-flanking region and 5′-UTR of human FCER1A. Additionally, reporter plasmids containing human FCER1A main promoter and 3′-UTR with all four possible combinations of +5650A>G and +5714G>A polymorphisms were constructed to evaluate functional potential of both 3′-UTR variants. However, no genotype-related differences in the gene expression were observed, as measured by reporter activity in cultured human basophil/mast cell-like KU812 cells, suggesting that both +5650A>G and +5714G>A have no genotype-related functional effect. In summary, we described linkage disequilibrium and the distribution of haplotypes for two identified human FCER1A 3′-UTR polymorphisms and several previously reported 5′-flanking region and 5′-UTR variants in Japanese and Poles, representative for East Asians and Caucasians, the two ethnic groups in which genetic associations between FCER1A and allergic diseases and/or serum IgE levels have been previously reported.
KeywordsFCER1A FcεRI Haplotype Linkage disequilibrium Polymorphism 3′-UTR
This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant (to D. P. P) and the Funding Program for Next Generation World-Leading Researchers from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to C. N.). We are grateful to Miss Michiyo Matsumoto for all her help and assistance.
- 3.Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Mitsuishi K, Ito T, Kawada H, Furukawa S, Ra C, Okumura K, Ogawa H (2003) A novel −66T/C polymorphism in FcεRI α-chain promoter affecting the transcription activity: possible relationship to allergic diseases. J Immunol 171:1927–1933PubMedGoogle Scholar
- 5.Kanada S, Nakano N, Potaczek DP, Maeda K, Shimokawa N, Niwa Y, Fukai T, Sanak M, Szczeklik A, Yagita H, Okumura K, Ogawa H, Nishiyama C (2008) Two different transcription factors discriminate the −315C>T polymorphism of the FcεRIα gene: binding of Sp1 to −315C and of a high mobility group-related molecule to −315T. J Immunol 180:8204–8210PubMedGoogle Scholar
- 26.Galanter J, Choudhry S, Eng C, Nazario S, Rodríguez-Santana JR, Casal J, Torres-Palacios A, Salas J, Chapela R, Watson HG, Meade K, LeNoir M, Rodríguez-Cintrón W, Avila PC, Burchard EG (2008) ORMDL3 gene is associated with asthma in three ethnically diverse populations. Am J Respir Crit Care Med 177:1194–1200PubMedCrossRefGoogle Scholar