Molecular Biology Reports

, Volume 39, Issue 3, pp 3343–3354

Estrogen related receptor α-induced adipogenesis is PGC-1β-dependent

  • Dapeng Ju
  • Jingjing He
  • Lili Zhao
  • Xueli Zheng
  • Gongshe Yang
Article

DOI: 10.1007/s11033-011-1104-8

Cite this article as:
Ju, D., He, J., Zhao, L. et al. Mol Biol Rep (2012) 39: 3343. doi:10.1007/s11033-011-1104-8

Abstract

Previous report showed that Estrogen related receptor α (ERRα) knockout mice had a significant reduction in adipose tissue deposition. Although it was reported that ERRα could promote adipogenesis in several immortalized preadipocytes cell lines, the mechanism behind which is still unclear to date. Besides, the expression pattern of ERRα in white adipose tissue is rarely examined. Here, we show that the expression of ERRα in primary cultured adipocytes is closely associated with adipogenesis. Besides, we found that peroxisome proliferator-activated receptor-γ coactivator 1β (PGC-1β) play an important role in regulating ERRα-induced adipogenesis. ERRα-induced adipogenesis was greatly attenuated when knocking down PGC- expression, while rescued by overexpression of PGC-. However, PGC-1β could still promote adipogenesis when suppressing ERRα expression. Furthermore, we demonstrated that ERRα could transcriptionally active PGC- expression and then enhance the formation of sterol-regulatory-element-binding protein-1c (SREBP-1c)/PGC-1β complex and peroxisome proliferator-activated receptor-γ (PPARγ)/PGC-1β complex. Taken together, these results indicate that ERRα-induced adipogenesis is triggered by modulating the expression of PGC-1β.

Keywords

ERRα PGC-1β Adipogenesis Primary adipocytes 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Dapeng Ju
    • 1
  • Jingjing He
    • 1
  • Lili Zhao
    • 1
  • Xueli Zheng
    • 1
  • Gongshe Yang
    • 1
  1. 1.Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and TechnologyNorthwest A&F UniversityYanglingPeople’s Republic of China

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