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Molecular Biology Reports

, Volume 39, Issue 3, pp 3225–3233 | Cite as

In vivo study of hepatitis B vaccine effects on inflammation and metabolism gene expression

  • Heyam Hamza
  • Jianhua Cao
  • Xinyun Li
  • Shuhong ZhaoEmail author
Article

Abstract

Pharmaceutical companies usually perform safety testing of vaccines, but all requirements of the World Health Organization and drug pharmacopoeias depend on general toxicity testing, and the gene expression study of hepatitis B vaccine is not done routinely to test vaccine quality. In this study, we applied a new technique of gene expression analysis to detect the inflammation and metabolism genes that might be affected by hepatitis B vaccine in mouse liver. Mice were used and divided into three groups: the first and second groups were treated with one or two human doses of vaccine, respectively, and the third group was used as a control. A microarray test showed that expression of 144 genes in the liver was significantly changed after 1 day of vaccination. Seven of these genes, which were related to inflammation and metabolism, were chosen and confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) at 1, 4 and 7 days. The expression level of these genes can be considered as a biomarker for the effects of the vaccine.

Keywords

Hepatitis B vaccine Inflammation genes Metabolism genes DNA microarray Quantitative RT-PCR analysis 

Notes

Acknowledgments

This research was supported by key project of National Natural Science Foundation of China (U0631005). We thank Yang Feng, GU Ting, Wong Feng Li and Shen He in our lab for sample collections and suggestions on data analyses.

Supplementary material

11033_2011_1090_MOESM1_ESM.doc (96 kb)
Supplementary material 1 (DOC 96 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Heyam Hamza
    • 1
  • Jianhua Cao
    • 1
  • Xinyun Li
    • 1
  • Shuhong Zhao
    • 1
    Email author
  1. 1.Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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