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Molecular Biology Reports

, Volume 39, Issue 3, pp 3137–3142 | Cite as

Characterization, expression profile, polymorphism and association of porcine NAT9 gene

  • Zhao Jiugang
  • Lan Jing
  • Liu Yonggang
Article
  • 210 Downloads

Abstract

The N-acetyltransferase 9 (NAT9) is an important reproduction related gene. In this study, we cloned the full-length cDNA sequence of porcine NAT9 gene through the rapid amplification of cDNA ends method. The porcine NAT9 gene encodes a protein of 206 amino acids which shares high homology with the NAT9 of seven species: rhesus monkey (87%), chimpanzee (87%), human (87%), horse (86%), rat (80%), mouse (79%) and gray short-tailed opossum (79%). This gene is structured in six exons and five introns as revealed by computer-assisted analysis. Tissue transcription profile analysis indicated that the porcine NAT9 gene is generally but differentially expressed in the detected tissues including spleen, large intestine, lung, fat, muscle, heart, liver, kidney and ovary. PCR-Alu I-RFLP was established to detect an A/G mutation on the position of 699-bp of mRNA and eight pig breeds display obvious genotype and allele frequency differences at this mutation locus. Association of this SNP with litter size traits was assessed in Large White (n = 100) and Landrace (n = 100) pig populations, and results demonstrated that this polymorphic locus is significantly associated with the litter size of first parity (P < 0.05). This suggests that the NAT9 gene can be an useful candidate gene in selection for increasing litter size in pigs.

Keywords

Pig NAT9 Tissue transcription profile Polymorphism Reproductive traits 

Notes

Acknowledgments

This work was supported by grants from the Natural Science Foundation Key Project of Yunnan Province (No. 2009CC015), National Nature Science Foundation of China (No. 30800810), Candidates of the Young and Middle Aged Academic and Technical Leaders of Yunnan Province (2009CI055), Applied Basic Research Project of Chongqing Academy of Animal Sciences (No. 10607) and Natural Science Foundation Project of CQ CSTC (2010BB1336).

Supplementary material

11033_2011_1079_MOESM1_ESM.doc (100 kb)
Supplementary material 1 (DOC 99 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Chongqing Key Laboratory of Pig Industry SciencesChongqing Academy of Animal SciencesRongchangChina
  2. 2.College of Animal Science and TechnologyYunnan Agricultural UniversityKunmingChina

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