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Molecular Biology Reports

, Volume 39, Issue 3, pp 2843–2849 | Cite as

Induction of cell differentiation and promotion of endocan gene expression in stomach cancer by melatonin

  • Sumei Zhang
  • Li Zuo
  • Shuyu Gui
  • Qing Zhou
  • Wei Wei
  • Yuan WangEmail author
Article

Abstract

The pineal hormone melatonin has been shown to have anticancer therapeutic properties in patients with gastric cancer, the mechanisms, however, remain largely unknown. The present study examined the effects of melatonin on cell differentiation related factors, namely, endocan, alkaline phosphatase, and lactate dehydrogenase, in gastric adenocarcinoma cell line SGC7901. Expression of endocan was significantly decreased in tissue of gastric cancer as compared to normal stomach tissue, as determined by immunohistochemical staining, and there is correlation between the degree of the decrease of endocan expression and the degree of differentiation of the cancer. Treatment of cultured gastric adenocarcinoma cells with 10−4 mol/l melatonin significantly increased the gene expression of endocan and down-regulated the activity of alkaline phosphatase and lactate dehydrogenase, two enzymes that promote de-differentiation in gastric tissue; and there was a negative correlation between the level of endocan expression and the activities of differentiation marker enzymes in the melatonin treated cancer cells. Gastric cancer cells treated with melatonin show more differentiated morphologic phenotype as compared the untreated cells. The findings indicate that melatonin may play its anticancer role in gastric adenocarcinoma by acting as a differentiation inducer.

Keywords

Gastric cancer SGC7901 Differentiation Endocan 

Notes

Acknowledgments

This study was supported by Natural Science Foundation of Anhui Province (No. 090413116) and National Natural Science Foundation of China (No. 30971226).

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Sumei Zhang
    • 1
    • 2
    • 4
  • Li Zuo
    • 1
    • 2
    • 4
  • Shuyu Gui
    • 3
  • Qing Zhou
    • 2
    • 4
  • Wei Wei
    • 1
  • Yuan Wang
    • 1
    • 2
    • 4
    Email author
  1. 1.Institute of Clinical PharmacologyAnhui Medical UniversityHefeiPeople’s Republic of China
  2. 2.Laboratory of Molecular Biology and Department of BiochemistryAnhui Medical UniversityHefeiPeople’s Republic of China
  3. 3.Department of Respiratory DiseaseAnhui Medical UniversityHefeiPeople’s Republic of China
  4. 4.Key Laboratory of Anti-inflammatory and Immunological Pharmacology, Ministry of Education and Key Laboratory of Gene Resource Utilization for Severe Disease of Anhui ProvinceAnhui Medical UniversityHefeiPeople’s Republic of China

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