Molecular Biology Reports

, Volume 39, Issue 3, pp 2439–2445 | Cite as

Molecular cloning, promoter analysis, SNP detection of Clusterin gene and their associations with mastitis in Chinese Holstein cows

  • Zeying Wang
  • Jingmin Huang
  • Jifeng Zhong
  • Genlin Wang


To examine the effect of Clusterin (CLU) on mastitis, genetic association analysis was applied on mastitis and milk production traits of 1,137 Chinese Holstein cows. We detected two novel single nucleotide polymorphisms (SNPs), G+15781A in the seventh exon and C-994T before 5′-upstream region (UTR) of CLU gene, found five TATA box, one CpG island and more transcription factor binding sites in promoter region, respectively, Milk fat rate in genotype AA was significantly higher than in GG on fat rate (P < 0.01), milk fat and milk yield in combined haplotype H1H4 (AGCT) were significantly higher than in H3H4 (GGCT) (P < 0.05), H1H4 was decided advantage in nine milk production traits. Quadruplet cows in G+15781A were decided advantage in each milk production traits that 305-day milk yield, fat rate, protein rate and somatic cell scores (SCS) increased following with birth order. A allele and T allele had positive effect on SCS. In conclusion, this study showed that the haplotype AA may be a genetic marker on mastitis and other performance for Chinese Holstein cows.


Clusterin Promoter SNP Haplotype Milk production traits Mastitis 



This research was supported by the Science and Technology Sustentation Project of China (2006BAD04A01, 2006BAD04A12), the Science and Technology Sustentation Project of Jiangsu (BE2008306-2), the 863 High Technology and Development Project of China (2007AA10Z169) and the High Technology Independent Innovation Project from Shandong Academy of Agricultural Sciences (No. 2007YCX019).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Center of Dairy Cattle Science ResearchShandong Academy of Agricultural SciencesJinanPeople’s Republic of China

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