Molecular Biology Reports

, Volume 39, Issue 3, pp 2375–2383 | Cite as

DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India

  • S. S. Gaikwad
  • H. V. Ghate
  • S. S. Ghaskadbi
  • M. S. Patole
  • Y. S. Shouche


We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.


Western Ghats of India DNA barcoding Butterflies Nymphalidae Cytochrome c oxidase I 



We would like to thank the authorities of Modern college, Pune-411005 and The Director, NCCS, Pune-411007 for the facilities provided. We also thank Mr. Parag Ragnekar and Dr. Amol Patwardhan for providing some specimen. This study was supported by DBT (Department of Biotechnology), Govt. of India, Grant No-BT/PR8351/NDB/51/138/2006. SG also thanks DBT for providing fellowship. We would also like to thank the anonymous referees for their valuable comments.

Supplementary material

11033_2011_988_MOESM1_ESM.doc (322 kb)
Supplementary material 1 (DOC 373 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • S. S. Gaikwad
    • 1
  • H. V. Ghate
    • 1
  • S. S. Ghaskadbi
    • 2
  • M. S. Patole
    • 3
  • Y. S. Shouche
    • 3
  1. 1.Department of ZoologyModern CollegePuneIndia
  2. 2.Department of ZoologyUniversity of PunePuneIndia
  3. 3.National Center for Cell SciencesPuneIndia

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