Molecular Biology Reports

, Volume 39, Issue 2, pp 2021–2029 | Cite as

The expression of HIF-1α in primary hepatocellular carcinoma and its correlation with radiotherapy response and clinical outcome

  • Zuo-Lin Xiang
  • Zhao-Chong Zeng
  • Jia Fan
  • Zhao-You Tang
  • Jian He
  • Hai-Ying Zeng
  • Joe Y. Chang
Article

Abstract

The purpose of this study was to evaluate the relationship between hypoxia-inducible factor-1α (HIF-1α) protein expression in hepatocellular carcinoma (HCC), and responses of abdominal metastatic lymph nodes (LNs) from HCC patients treated with external beam radiotherapy (EBRT). HIF-1α immunohistochemical staining was performed on tissue microarrays (TMAs) of primary HCC specimens from 69 HCC patients with abdominal LN metastases. All patients received abdominal metastatic LN EBRT at the Department of Radiation Oncology at Zhongshan Hospital. A receiver-operating characteristic (ROC)-based approach and logistical regression analysis were used to determine the predictive value of HIF-1α expression in primary tumors with HCC metastatic LN EBRT response. Kaplan–Meier curves and log-rank tests were used to analyze patient survival. Cox proportional hazards regression model was used to analyze independent prognostic factors. HIF-1α expression was correlated with blood hemoglobin (Hb: r = −0.280, P = 0.020), response of abdominal metastatic LNs to EBRT (r = 0.286, P = 0.017), locoregional recurrence (r = 0.278, P = 0.021), and cancer-specific deaths (r = 0.298, P = 0.013). HIF-1α expression was predictive of EBRT response of metastatic LNs [area under the curve (AUC): 0.646; 95% confidence interval (CI): 0.499–0.793; P = 0.047], locoregional recurrence (AUC: 0.657; 95% CI: 0.509–0.805; P = 0.049) and cancer-specific deaths (AUC: 0.671; 95% CI: 0.531–0.812; P = 0.035). Patients with tumors exhibiting high HIF-1α expression had significantly poorer overall survival (OS) than those with low tumor expression of HIF-1α (P = 0.016). Multivariate analysis showed that Hb (P = 0.035), vascular invasion (P = 0.026), Child-Pugh score (P < 0.001), intrahepatic tumor control (P < 0.001), and HIF-1α (P = 0.020) were independent prognosis factors for OS of HCC patients after receiving abdominal metastatic LN EBRT. HIF-1α expression in primary HCCs was associated with EBRT response of abdominal metastatic LNs and poor prognosis.

Keywords

Abdominal lymph nodes External beam radiotherapy Hepatocellular carcinoma Immunohistochemistry Tissue microarray 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zuo-Lin Xiang
    • 1
  • Zhao-Chong Zeng
    • 1
  • Jia Fan
    • 2
  • Zhao-You Tang
    • 2
  • Jian He
    • 1
  • Hai-Ying Zeng
    • 3
  • Joe Y. Chang
    • 4
  1. 1.Department of Radiation Oncology, Zhongshan HospitalFudan UniversityShanghaiChina
  2. 2.Liver Cancer Institute, Zhongshan HospitalFudan UniversityShanghaiChina
  3. 3.Department of Pathology, Zhongshan HospitalFudan UniversityShanghaiChina
  4. 4.Department of Radiation OncologyThe University of Texas M. D. Anderson Cancer CenterHoustonUSA

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