Molecular Biology Reports

, Volume 39, Issue 2, pp 1823–1829 | Cite as

Association of pig UCP3 gene mutations and back fat thickness in the sixth and seventh rib

  • Hongxia Li
  • Olafemi Hermann Dave Brahi
  • Xingbo ZhaoEmail author
  • Ningying Xu
  • Xiaofeng Zhao


Accumulated studies have documented extensive links between UCP3 polymorphisms and pig productive traits, and quantitative trait loci linkage results, on the other hand, provided extensive evidences showing that UCP3 was in the core of several QTLs for carcass and meat quality traits. In this research, we screened two substitutions in coding sequence and one 9-base continuous mutated site in 3′UTR of pig UCP3 gene using the reference population of 293 pigs which were F 2 generation of hybrids between Chinese native Jinhua pigs and European Pietrain. The two missense mutations of G1406A in Exon 3 and T3602C in Exon 5 which led to changes in the G150R and M259T, respectively, were digested by SmaI and introduced Tth111I separately for genotype analysis, and the 9-base continuous mutated site in the 3′UTR was analyzed by an AvaI cleavage. As a result, the 9-base continuous mutated site of 3′UTR manifested significantly close association with the backfat thickness at the sixth and seventh rib, but the polymorphisms of G1406A and T3602C were not associated significantly with any of the seven carcass traits. The same results were shown by RT-qPCR and western blotting. These findings inferred that UCP3 probably has tissue-specific effects on pig carcass traits.


Backfat thickness Mutation Genotype Pig UCP3 



This work was supported by the Domain-Specific Projects for transgenic biological breeding (2008ZX08009-002 and 2009ZX08009-157B). We thank Dr. Juming Zhong in Aubum University for improving the manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Hongxia Li
    • 1
    • 3
  • Olafemi Hermann Dave Brahi
    • 1
  • Xingbo Zhao
    • 1
    Email author
  • Ningying Xu
    • 2
  • Xiaofeng Zhao
    • 2
  1. 1.State Key Laboratory for Agrobiotechnology, Key Laboratory of Animal Genetics and Breeding of Ministry of AgricultureChina Agricultural UniversityBeijingChina
  2. 2.College of Animal ScienceZhejiang UniversityHangzhouChina
  3. 3.Dalian Medical UniversityDalianChina

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