Advertisement

Molecular Biology Reports

, Volume 39, Issue 2, pp 1823–1829 | Cite as

Association of pig UCP3 gene mutations and back fat thickness in the sixth and seventh rib

  • Hongxia Li
  • Olafemi Hermann Dave Brahi
  • Xingbo ZhaoEmail author
  • Ningying Xu
  • Xiaofeng Zhao
Article

Abstract

Accumulated studies have documented extensive links between UCP3 polymorphisms and pig productive traits, and quantitative trait loci linkage results, on the other hand, provided extensive evidences showing that UCP3 was in the core of several QTLs for carcass and meat quality traits. In this research, we screened two substitutions in coding sequence and one 9-base continuous mutated site in 3′UTR of pig UCP3 gene using the reference population of 293 pigs which were F 2 generation of hybrids between Chinese native Jinhua pigs and European Pietrain. The two missense mutations of G1406A in Exon 3 and T3602C in Exon 5 which led to changes in the G150R and M259T, respectively, were digested by SmaI and introduced Tth111I separately for genotype analysis, and the 9-base continuous mutated site in the 3′UTR was analyzed by an AvaI cleavage. As a result, the 9-base continuous mutated site of 3′UTR manifested significantly close association with the backfat thickness at the sixth and seventh rib, but the polymorphisms of G1406A and T3602C were not associated significantly with any of the seven carcass traits. The same results were shown by RT-qPCR and western blotting. These findings inferred that UCP3 probably has tissue-specific effects on pig carcass traits.

Keywords

Backfat thickness Mutation Genotype Pig UCP3 

Notes

Acknowledgments

This work was supported by the Domain-Specific Projects for transgenic biological breeding (2008ZX08009-002 and 2009ZX08009-157B). We thank Dr. Juming Zhong in Aubum University for improving the manuscript.

References

  1. 1.
    Criscuolo F, Mozo J, Hurtaud C et al (2006) UCP2, UCP3, avUCP, what do they do when proton transport is not stimulated? Possible relevance to pyruvate and glutamine metabolism. Biochim Biophys Acta 1757(9–10):1284–1291PubMedGoogle Scholar
  2. 2.
    Enerback S, Jacobsson A, Simpson EM et al (1997) Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. Nature 387(6628):90–94PubMedCrossRefGoogle Scholar
  3. 3.
    Mao W, Yu XX, Zhong A et al (1999) UCP4, a novel brain-specific mitochondrial protein that reduces membrane potential in mammalian cells. FEBS Lett 443(3):326–330PubMedCrossRefGoogle Scholar
  4. 4.
    Sanchis D, Fleury C, Chomiki N et al (1998) BMCP1, a novel mitochondrial carrier with high expression in the central nervous system of humans and rodents, and respiration uncoupling activity in recombinant yeast. J Biol Chem 273(51):34611–34615PubMedCrossRefGoogle Scholar
  5. 5.
    Fleury C, Neverova M, Collins S et al (1997) Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat Genet 15(3):269–272PubMedCrossRefGoogle Scholar
  6. 6.
    Damon M, Louveau I, Lefaucheur L et al (2006) Number of intramuscular adipocytes and fatty acid binding protein-4 content are significant indicators of intramuscular fat level in crossbred Large White × Duroc pigs. J Anim Sci 84(5):1083–1092PubMedGoogle Scholar
  7. 7.
    Mostyn A, Litten JC, Perkins KS et al (2004) Influence of genotype on the differential ontogeny of uncoupling protein 2 and 3 in subcutaneous adipose tissue and muscle in neonatal pigs. J Endocrinol 183(1):121–131PubMedCrossRefGoogle Scholar
  8. 8.
    Mostyn A, Litten JC, Perkins KS et al (2005) Influence of size at birth on the endocrine profiles and expression of uncoupling proteins in subcutaneous adipose tissue, lung, and muscle of neonatal pigs. Am J Physiol Regul Integr Comp Physiol 288(6):R1536–R1542PubMedCrossRefGoogle Scholar
  9. 9.
    Werner P, Neuenschwander S, Stranzinger G (1999) Characterization of the porcine uncoupling proteins 2 and 3 (UCP2 & UCP3) and their localization to chromosome 9 p by somatic cell hybrids. Anim Genet 30(3):221–224PubMedCrossRefGoogle Scholar
  10. 10.
    Damon M, Vincent A, Lombardi A et al (2000) First evidence of uncoupling protein-2 (UCP-2) and -3 (UCP-3) gene expression in piglet skeletal muscle and adipose tissue. Gene 246(1–2):133–141PubMedCrossRefGoogle Scholar
  11. 11.
    Knoll A, Putnova L, Dvorak J et al (2000) Linkage mapping of an AvaI PCR-RFLP within the porcine uncoupling protein 3 (UCP3) gene. Anim Genet 31(2):156–157PubMedCrossRefGoogle Scholar
  12. 12.
    Fang M, Zhao X, Li N et al (2002) Genetic analysis on 3′-terminal flanking region of uncoupling protein 3 in different pig breeds. Chin Sci Bull 147:1541–1543CrossRefGoogle Scholar
  13. 13.
    Li H, Li Y, Zhao X et al (2005) Structure and nucleotide polymorphisms in pig uncoupling protein 2 and 3 genes. Anim Biotechnol 16(2):209–220PubMedCrossRefGoogle Scholar
  14. 14.
    Liu BH (1998) Statistical genomics: linkage, mapping and QTL analysis. CRC Press, Boca RatonGoogle Scholar
  15. 15.
    Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucl Acids Res 31(13):3406–3415PubMedCrossRefGoogle Scholar
  16. 16.
    de Moor CH, Meijer H, Lissenden S (2005) Mechanisms of translational control by the 3′UTR in development and differentiation. Semin Cell Dev Biol 16(1):49–58PubMedCrossRefGoogle Scholar
  17. 17.
    Hughes TA (2006) Regulation of gene expression by alternative untranslated regions. Trends Genet 22(3):119–122PubMedCrossRefGoogle Scholar
  18. 18.
    Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136(2):215–233PubMedCrossRefGoogle Scholar
  19. 19.
    Evans GJ, Giuffra E, Sanchez A et al (2003) Identification of quantitative trait loci for production traits in commercial pig populations. Genetics 164(2):621–627PubMedGoogle Scholar
  20. 20.
    Vidal O, Noguera JL, Amills M et al (2005) Identification of carcass and meat quality quantitative trait loci in a Landrace pig population selected for growth and leanness. J Anim Sci 83(2):293–300PubMedGoogle Scholar
  21. 21.
    Edwards DB, Ernst CW, Tempelman RJ et al (2008) Quantitative trait loci mapping in an F2 Duroc × Pietrain resource population: I. Growth traits. J Anim Sci 86(2):241–253PubMedCrossRefGoogle Scholar
  22. 22.
    Katsumata M, Matsumoto M, Kawakami S et al (2004) Effect of heat exposure on uncoupling protein-3 mRNA abundance in porcine skeletal muscle. J Anim Sci 82(12):3493–3499PubMedGoogle Scholar
  23. 23.
    Ramsay TG, Mitchell AD (2008) Impact of dietary protein content on uncoupling protein mRNA abundance in swine. Comp Biochem Physiol B 149(4):562–571PubMedCrossRefGoogle Scholar
  24. 24.
    Costford SR, Chaudhry SN, Salkhordeh M et al (2006) Effects of the presence, absence, and overexpression of uncoupling protein-3 on adiposity and fuel metabolism in congenic mice. Am J Physiol Endocrinol Metab 290(6):E1304–E1312PubMedCrossRefGoogle Scholar
  25. 25.
    Ren L, Zhu B, Han D et al (2006) Polymorphism of porcine uncoupling protein 3(UCP3) and its association with fat deposition and meat quality. J Agric Biotechnol 14:652–656Google Scholar
  26. 26.
    Lanouette CM, Chagnon YC, Rice T et al (2002) Uncoupling protein 3 gene is associated with body composition changes with training in HERITAGE study. J Appl Physiol 92(3):1111–1118PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Hongxia Li
    • 1
    • 3
  • Olafemi Hermann Dave Brahi
    • 1
  • Xingbo Zhao
    • 1
    Email author
  • Ningying Xu
    • 2
  • Xiaofeng Zhao
    • 2
  1. 1.State Key Laboratory for Agrobiotechnology, Key Laboratory of Animal Genetics and Breeding of Ministry of AgricultureChina Agricultural UniversityBeijingChina
  2. 2.College of Animal ScienceZhejiang UniversityHangzhouChina
  3. 3.Dalian Medical UniversityDalianChina

Personalised recommendations