Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus
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The four tropane alkaloids have played a pivotal role in controlling diseases such as the toxic and septic shock, the organophosphorus poison and the acute lung injury. Here, the elicitation effect of different elicitors on the production of tropane alkaloids and the molecular mechanism of enzyme genes in the pathway was firstly demonstrated in hairy roots of Anisodus acutangulus. The results showed ethanol, methyl jasmonate and Ag+ could improve the accumulation of tropane alkaloids up to 1.51, 1.13 and 1.08 times after 24 h treatment, respectively (P < 0.05), whereas salicylic acid decreased the average content of tropane alkaloids. Furthermore, expression profile analysis results revealed that up-regulation of hyoscyamine-6b-hydroxylase (AaH6H) and little regulation of tropinone reducase II (AaTR2) elicited by ethanol, increased expression of putrescine N-methyltransferase I (AaPMT1) elicited by Ag+, elevated expression of tropinone reducase I (AaTR1) elicited by methyl jasmonate, respectively, resulted in tropane alkaloids improvement. Our results showed that hairy root culture of A. acutangulus in combination with elicitors was a promising way for production of tropane alkaloids in the future.
KeywordsAnisodus acutangulus Hairy roots Tropane alkaloids Elicitors Biosynthetic genes
- A. acutangulus
Salicylic acid dissolved in ethanol
Reverse transcriptase-polymerase chain reaction
This work was supported by National Natural Science Fund (30900110), Shanghai Science and Technology Committee Project (10JC1412000, 09QH1401900, 06QA14038, 08391911800, 073158202, 075405117, 065458022, 05ZR14093), Shanghai Education Committee Fund (09ZZ138, 06DZ015, J50401), Zhejiang Provincial Natural Science Fund (Y2080621), Fujian Science and Technology Committee Key Special Project (2008NZ0001-4), National Transgenic Organism New Variety Culture Key Project (2009ZX08012-002B), Project from Shanghai Normal University (SK200830, CH030). Miss Pin Liu’s (Shanghai Jiaotong University, China) kind assistance with HPLC analysis is also acknowledged.
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