Molecular Biology Reports

, Volume 39, Issue 2, pp 1315–1322

Characterization of acute renal allograft rejection by proteomic analysis of renal tissue in rat

  • Gang Chen
  • Jing-bin Huang
  • Jie Mi
  • Yun-feng He
  • Xiao-hou Wu
  • Chun-li Luo
  • Si-min Liang
  • Jia-bing Li
  • Ya-xiong Tang
  • Jie Li
Article

DOI: 10.1007/s11033-011-0864-5

Cite this article as:
Chen, G., Huang, J., Mi, J. et al. Mol Biol Rep (2012) 39: 1315. doi:10.1007/s11033-011-0864-5

Abstract

Rapid and reliable biomarkers of renal allograft rejection have not been available. This study aimed to investigate biomarkers in renal allograft tissue using proteomic analysis. Orthotopic kidney transplantations were performed using Fisher (F344) or Lewis rats as donors and Lewis rats as recipients. Syngenic control group (Group I) constituted F344-to-F344 orthotopic kidney allo-transplantations (n = 8); and allogenic group (Group II) consisted of F344-to-Lewis orthotopic kidney allo-transplantations (n = 8). Renal tissues were harvested 7 days after transplantation. Samples were analyzed using 2-D electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry. 6 differentially expressed proteins were identified between allogenic group and syngenic control group. A rat model of acute renal allograft rejection was successfully set up. Differentially expressed proteins in renal allograft tissue of rat were detected using proteomic analysis and might serve as novel diagnostic and therapeutic targets in human. Quantitative proteomics, using MALDL-TOF-MS methodology has the potential to provide a profiling and a deeper understanding of acute renal rejection.

Keywords

Renal allograft rejection Proteomic analysis Rat 

Abbreviations

IPG

Immobilized pH gradient

MALDL-TOF-MS

Matrix assisted laser desorption ionization-time of flight mass spectrometry

PMF

Peptide mapping fingerprints

PAGE

Polyacrylamide gel electrophoresis

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Gang Chen
    • 1
  • Jing-bin Huang
    • 2
  • Jie Mi
    • 3
  • Yun-feng He
    • 1
  • Xiao-hou Wu
    • 1
  • Chun-li Luo
    • 4
  • Si-min Liang
    • 1
  • Jia-bing Li
    • 1
  • Ya-xiong Tang
    • 1
  • Jie Li
    • 1
  1. 1.Department of UrologyThe First Affiliated Hospital, Chongqing Medical UniversityChongqingChina
  2. 2.Department of Cardiothoracic SurgeryThe Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical CollegeNanningChina
  3. 3.Emergency DepartmentThe First Affiliated Hospital, Chongqing Medical UniversityChongqingChina
  4. 4.Clinical Laboratory MedicineThe First Affiliated Hospital, Chongqing Medical UniversityChongqingChina

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