Molecular Biology Reports

, Volume 39, Issue 2, pp 1087–1093 | Cite as

The expression of damage-regulated autophagy modulator 2 (DRAM2) contributes to autophagy induction

  • Jung-Ho Yoon
  • Song Her
  • Moonhee Kim
  • Ik-Soon Jang
  • Junsoo ParkEmail author


Autophagy is a membrane trafficking process involved in intracellular degradation and recycling in eukaryotic cells. DRAM2 (damage-regulated autophagy modulator 2) is a homologue of DRAM that regulates p53-mediated cell death. As its name implies, DRAM expression induces autophagy in a p53-dependent manner; however, the role of DRAM2 in autophagy is not clear. In this study, we report that DRAM2 expression contributes to autophagy induction. Overexpression of DRAM2 induces cytoplasmic GFP-LC3 punctuates, and increases the level of endogenous LC3-II. Moreover, the silencing of endogenous DRAM2 interferes with starvation-induced autophagy. Thus, we propose that DRAM2 as well as DRAM are involved in autophagy.


DRAM2 DRAM Autophagy Starvation LC3 



We would particularly like to thank Kevin M. Ryan for providing the DRAM expression plasmid and T. Yoshimori for providing the GFP-LC3 plasmid. This study was supported by a National Research Foundation of Korea Grant funded by the Korean Government (2010-0009567 & 2009-0065887) and by the Mid-career Researcher Program (No. RO1-2008-000-20253-0).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Jung-Ho Yoon
    • 1
  • Song Her
    • 2
  • Moonhee Kim
    • 1
  • Ik-Soon Jang
    • 3
  • Junsoo Park
    • 1
    • 4
    Email author
  1. 1.Division of Biological Science and TechnologyYonsei UniversityWonjuRepublic of Korea
  2. 2.Korea Basic Science Institute, Chuncheon CenterChuncheonRepublic of Korea
  3. 3.Division of Life ScienceKorea Basic Science InstituteDaejeonRepublic of Korea
  4. 4.Division of Biological Sciences and Technology, Yonsei Institute of Space BioscienceYonsei UniversityWonjuRepublic of Korea

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