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Molecular Biology Reports

, Volume 38, Issue 5, pp 2939–2943 | Cite as

Association of the colorectal cancer and MDR1 gene polymorphism in an Iranian population

  • Azam Khedri
  • Amireh Nejat-Shokouhi
  • Roham Salek
  • Habibollah Esmaeili
  • Ali Mokhtarifar
  • Reza Entezari Heravi
  • Farnoosh Tatari
  • Javad BehravanEmail author
  • Behnoosh Miladpour
  • Shahireh Omidvar Tehrani
Article

Abstract

The human multidrug resistance (MDR1) gene product P-glycoprotein is highly expressed in intestinal epithelial cells, where it constitutes a barrier against xenobiotics, bacterial toxins, drugs and other biologically active compounds, possibly carcinogens. In this study, an association of MDR1 gene polymorphism and the occurrence of colorectal cancer were evaluated. In this case-control-designed 118 unrelated colorectal cancer and 137 sex-and-ages matched healthy controls were enrolled. The C3435T MDR1 gene polymorphism was identified using the polymerase chain reaction-restriction fragment length polymorphism method. Significantly increased frequencies of the 3435T allele and the 3435TT were observed in patients with colorectal cancer compared with controls (P = 0.03; OR, 95% CI; 1.46 for 3435T allele and P = 0.003; OR, 95% CI; 2.2 for 3435TT genotype). In contrast, frequency of genotype TT was significantly higher in controls compared to colorectal cancer (P = 0.006; OR, 95% CI; 0.49 for TC genotype). In this study suggest that C3435T MDR1 polymorphism has an association with colorectal cancer. The results support that the presence of allele C results in decreased susceptibility to colorectal cancer.

Keywords

Colorectal cancer MDR1 P-gp C3435T polymorphism 

Notes

Acknowledgemnt

The authors are indebted to the research council, Mashhad University of Medical Sciences, Iran, for approval and financial support of this project.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Azam Khedri
    • 1
  • Amireh Nejat-Shokouhi
    • 2
  • Roham Salek
    • 3
  • Habibollah Esmaeili
    • 4
  • Ali Mokhtarifar
    • 5
  • Reza Entezari Heravi
    • 6
  • Farnoosh Tatari
    • 6
  • Javad Behravan
    • 6
    • 7
    Email author
  • Behnoosh Miladpour
    • 8
  • Shahireh Omidvar Tehrani
    • 8
  1. 1.Islamic Azad University Branch MasjedsoleimanMasjedsoleimanIran
  2. 2.Department of Clinical BiochemistryMashhad University of Medical SciencesMashhadIran
  3. 3.Omid and Imam Reza Hospital, Cancer Research CenterMashhad University of Medical SciencesMashhadIran
  4. 4.Department of Medical StatisticsMashhad University of Medical SciencesMashhadIran
  5. 5.Department of Internal MedicineImam Reza Hospital, Mashhad University of Medical SciencesMashhadIran
  6. 6.Biotechnology Research Center, School of PharmacyMashhad University of Medical SciencesMashhadIran
  7. 7.Department of Pharmaceutical BiotechnologySchool of PharmacyMashhadIran
  8. 8.Biotechnology Research CenterMashhad University of Medical SciencesMashhadIran

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