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Molecular Biology Reports

, Volume 38, Issue 8, pp 5055–5063 | Cite as

Proteomic response of barley leaves to salinity

  • Abdolrahman Rasoulnia
  • Mohammad Reza Bihamta
  • Seyed Ali Peyghambari
  • Houshang Alizadeh
  • Afrasyab Rahnama
Article

Abstract

Drought and salinity stresses are adverse environmental factors that affect crop growth and yield. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in living system. We applied this technique to investigate protein changes that were induced by salinity in barley genotypes (Hordeum vulgare L.), Afzal, as a salt-tolerant genotype and L-527, as a salt-sensitive genotype. The seeds of two genotypes were sown in pot under controlled condition of greenhouse, using a factorial experiment based on a randomized complete block design with three replications. Salt stress was imposed at seedling stage and leaves were collected from control and salt-stressed plant. The Na+ and K+ concentrations in leaves changed significantly in response to short-term stress. About 850 spots were reproducibly detected and analyzed on 2-DE gels. Of these, 117 proteins showed significant change under salinity condition in at least one of the genotypes. Mass spectrometry analysis using MALDI-TOF/TOF led to the identification some proteins involved in several salt responsive mechanisms which may increase plant adaptation to salt stress including higher constitutive expression level and upregulation of antioxidant, upregulation of protein involved in signal transduction, protein biosynthesis, ATP generation and photosynthesis. These findings may enhance our understanding of plant molecular response to salinity.

Keywords

Barley 2-D electrophoresis Proteomics Salinity 

Notes

Acknowledgment

We are grateful to Professor Claire Gay, York university of UK, facility for performing the MS analysis and associated bioinformatics.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Abdolrahman Rasoulnia
    • 1
  • Mohammad Reza Bihamta
    • 1
  • Seyed Ali Peyghambari
    • 1
  • Houshang Alizadeh
    • 1
  • Afrasyab Rahnama
    • 2
  1. 1.Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural SciencesUniversity of TehranKarajIran
  2. 2.Department of Agronomy and Plant Breeding, College of AgricultureShahid Chamran UniversityAhvazIran

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