Molecular Biology Reports

, Volume 38, Issue 8, pp 4965–4969 | Cite as

Effect of genetic variation of CEBPA gene on body measurement and carcass traits of Qinchuan cattle



In our study, genetic variation in coding region of cattle CCAAT enhancer binding protein alpha(namely CEBPA)gene was detected by PCR-RFLP and DNA sequencing methods in 215 individuals from Qinchuan cattle breed. Two haplotypes (A and B) and three observed genotypes (AA, AB, and BB) were detected. The result of DNA sequence showed one mutation by comparisons with NC_007316. The mutation at nt963 (T>G) were located in coding region of the CEBPA gene. Associations between the CEBPA gene genetic variation and the carcass traits were revealed in Qinchuan cattle. Least squares analysis revealed a significant statistical effect of the CEBPA gene different genotypes on slaughter weight and carcass weight in Qinchuan cattle. Individuals with BB genotype showed higher slaughter weight and carcass weight than individuals with AA and AB genotypes. Therefore, these results suggest that the CEBPA gene is a strong candidate gene that affects carcass traits in Qinchuan cattle.


CEBPA gene Genetic variation PCR-RFLP Carcass traits Qinchuan cattle 



This study was supported by the National “863” Program of China (No. 2008AA101010), “13115” Sci-Tech Innovation Program of Shaanxi Province (2008ZDKG-11).


  1. 1.
    Ramji DP, Foka P (2002) CCAAT/enhancer-binding proteins: structure, function and regulation. Biochem J 365:561–575PubMedGoogle Scholar
  2. 2.
    Agre P, Johnson PF, McKnight SL (1989) Cognate DNA binding specificity retained after leucine zipper exchange between GCN4 and C/EBP. Science 246(4932):922–926PubMedCrossRefGoogle Scholar
  3. 3.
    Vinson CR, Hai T, Boyd SM (1993) Dimerization specificity of the leucine zipper-containing bZIP motif on DNA binding: prediction and rational design. Genes Dev 7:1047–1058PubMedCrossRefGoogle Scholar
  4. 4.
    Landschulz WH, Johnson PF, McKnight SL (1988) The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 240(4860):1759–1764PubMedCrossRefGoogle Scholar
  5. 5.
    Vinson CR, Sigler PB, McKnight SL (1989) Scissors-grip model for DNA recognition by a family of leucine zipper proteins. Science 246:911–916PubMedCrossRefGoogle Scholar
  6. 6.
    Hurst HC (1995) Transcription factors 1: bZIP proteins. Protein Profile 2:101–168PubMedGoogle Scholar
  7. 7.
    Agre P, Johnson PF, McKnight SL (1989) Cognate DNA binding specificity retained after leucine zipper exchange between GCN4 and C/EBP. Science 246:922–926PubMedCrossRefGoogle Scholar
  8. 8.
    Johnson PF (1993) Identification of C/EBP basic region residues involved in DNA sequence recognition and half-site spacing preference. Mol Cell Biol 13:6919–6930PubMedGoogle Scholar
  9. 9.
    Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM (2000) Transcriptional regulation of adipogenesis. Genes Dev 14:1293–1307PubMedGoogle Scholar
  10. 10.
    McKnight SL, Lane MD, Gluecksohn-Waelsch S (1989) Is CCAAT/enhancer-binding protein a central regulator of energy metabolism? Genes Dev 3:2021–2024PubMedCrossRefGoogle Scholar
  11. 11.
    Hendricks-Taylor LR, Darlington GJ (1995) Inhibition of cell proliferation by C/EBPα occurs in many cell types, does not required the presence of p53 or Rb and is not affected by the large T antigen. Nucleic Acids Res 23:4726–4733PubMedCrossRefGoogle Scholar
  12. 12.
    Antonson P, Xanthopoulos KG (1995) Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). Biochem Biophys Res Commun 215:106–113PubMedCrossRefGoogle Scholar
  13. 13.
    Van Doorn SBVW, Meijer J et al (2003) Biallelic mutations in the CEBPA gene and low CEBPA expression levels as prognostic markers in intermediate risk AML. Hematol J 4:31–40CrossRefGoogle Scholar
  14. 14.
    Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissue. Trends Genet 5:391PubMedGoogle Scholar
  15. 15.
    Gilbert RP, Bailey DR, Shannon NH (1993) Linear body measurements of cattle before and after twenty years of selection for postweaning gain when fed two different diets. J Anim Sci 71:1712–1720PubMedGoogle Scholar
  16. 16.
    Huang YZ, Zhang EP, Chen H, Wang J, Li ZJ, Huai YT, Ma L, Lan XY, Ren G, Lei CZ, Fang XT, Wang JQ (2010) Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits. J Appl Genet 51(2):199–202PubMedCrossRefGoogle Scholar
  17. 17.
    Nei M, Roychoudhury AK (1974) Sampling variance of heterozygosity and genetic distance. Genetics 76:379–390PubMedGoogle Scholar
  18. 18.
    Nei M, Li WH (1979) Mathematic model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273PubMedCrossRefGoogle Scholar
  19. 19.
    Zhao Q, Davis ME, Hines HC (2004) Associations of polymorphisms in the Pit-1 gene with growth and carcass traits in Angus beef cattle. J Anim Sci 82:2229–2233PubMedGoogle Scholar
  20. 20.
    He H, Liu XL, Gu YL, Liu Y (2010) A novel 18-bp deletion mutation of the AMPD1 gene affects carcass traits in Qinchuan cattle. Mol Biol Rep 37:3945–3949Google Scholar
  21. 21.
    Zhang CL, Wang YH, Chen H, Lan XY, Lei CZ, Fang XT (2008) Association between variants in the 50-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle. Mol Biol Rep 36:1839–1843PubMedCrossRefGoogle Scholar
  22. 22.
    Fuchs O, Provaznikova D, Kocova M et al (2008) CEBPA polymorphisms and mutations in patients with acute myeloid leukemia, myelodysplastic syndrome, multiple myeloma and non-Hodgkin’s lymphoma. Blood Cells Mol Dis 40(3):401–405PubMedCrossRefGoogle Scholar
  23. 23.
    Gombart AF, Hofmann WK, Kawano S et al (2002) Mutations in the gene encoding the transcription factor CCAAT/enhancer binding protein α in myelodysplastic syndromes and myeloid leukemias. Blood 99:1332–1340PubMedCrossRefGoogle Scholar
  24. 24.
    Frohling S, Schlenk RF, Stolze I et al (2004) CEBPA mutations in younger adults with acute myeloid leukemia and normal cytogenetics: prognostic relevance and analysis of cooperating mutations. J Clin Oncol 22:624–633PubMedCrossRefGoogle Scholar
  25. 25.
    Lin LI, Chen CY, Lin DT et al (2005) Characterization of CEBPA mutations in acute myeloid leukemia: most patients with CEBPA mutations have biallelic mutations and show a distinct immunophenotype of the leukemic cells. Clin Cancer Res 11:1372–1379PubMedCrossRefGoogle Scholar
  26. 26.
    Liang DC, Shih LY, Juany CF et al (2005) CEBPα mutations in childhood acute myeloid leukemia. Leukemia 19:410–414PubMedCrossRefGoogle Scholar
  27. 27.
    Smith ML, Cavenagh JD, Lister TA, Fitzgibbon J (2005) Mutation of CEBPA in familial acute myeloid leukemia. N Engl J Med 351:2403–2407CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hua He
    • 1
  • Xiaolin Liu
    • 1
  • Yulan Gu
    • 1
  • Yu Liu
    • 1
  • Jing Yang
    • 1
  1. 1.College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for AgricultureNorthwest A&F UniversityYanglingPeople’s Republic of China

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