Molecular Biology Reports

, Volume 38, Issue 6, pp 3921–3928 | Cite as

Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15

Article

Abstract

Transcriptional regulation is thought to be important for stress tolerance and response of transcription factors. RAV subfamily transcription factor contains an AP2- and B3-DNA binding domain, which belongs to the AP2/ERF family. It encodes transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants. Here, a RAV-like gene, BnaRAV-1-HY15, was isolated from Brassica napus L. cv HuYou15. Sequence homology analysis revealed that the BnaRAV-1-HY15 factor belongs to the RAV subfamily of the AP2/ERF family, and it shares high identity with the AtRAV2 of Arabidopsis. Sequence and three-dimensional structural analyses revealed that BnaRAV-1-HY15 contains two distinct DNA-binding domains, one AP2 domain together with one B3 domain. The AP2 domain composed of 54 amino acids and present in N-terminal region. In addition to AP2 domain, 117 amino acids show significant sequence similarity to the B3 domain present in C-terminal region. Semi-quantitative RT-PCR analysis indicated that the BnaRAV-1-HY15 gene is induced by cold, NaCl and PEG treatments. Under ABA stress, the expression of BnaRAV-1-HY15 gene was not detected. The gene expression was also not traceable from the tissues of pod, bud, petal, leaf, stem and root of normally grown B. napus L. HuYou15 plant at the period of flowering and seed development.

Keywords

Transcription factor AP2 family BnaRAV-1-HY15 Abiotic stresses Brassica napus L. HuYou15 

Abbreviations

TF

Transcription factor

AP2

APETALA2 factor

ERF

Ethylene responsive element binding factor

DREB

Dehydration responsive element binding factor

RAV

Related to ABI3/VP

Hsp

Heat shock protein

bZIP

Basic leucine zipper

EST

Expressed sequence tag

ABA

Abscisic acid

Notes

Acknowledgements

The research was supported by Shanghai Natural Science Foundation (08ZR1417200), Shanghai Rising-Star Program and (08QH14021), International Scientific and Technological Cooperation of Shanghai-Alberta. The authors would like to thank Dr. Jeffrey Charrois and Andrea Bailey for providing the critical reading the manuscript and valuable suggestions.

Supplementary material

11033_2010_508_MOESM1_ESM.docx (559 kb)
Supplementary material 1 (DOCX 558 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Crop Breeding and Cultivation Research InstituteShanghai Academy of Agricultural SciencesShanghaiChina
  2. 2.Life Science, Alberta Innovates-Technology FuturesABCanada
  3. 3.Biotechnology Research InstituteShanghai Academy of Agricultural SciencesShanghaiChina

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