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Molecular Biology Reports

, Volume 38, Issue 6, pp 3839–3848 | Cite as

Analysis on DNA sequence of GPR54 gene and its association with litter size in goats

  • G. L. Cao
  • M. X. Chu
  • L. Fang
  • T. Feng
  • R. Di
  • N. Li
Article

Abstract

The kisspeptin/GPR54 pathway is crucial in the process of puberty onset. Six pairs of primers were designed to clone goat GPR54 and scan polymorphisms and one pair of primers to detect polymorphisms of GPR54 in sexual precocious and sexual late-maturing goat breeds. A DNA fragment of 4258 bp of goat GPR54 was obtained, which contains an open reading frame (ORF) of 1137 bp and encodes 378 amino acids, having a high homology with other mammals. The protein was predicted to have seven transmembrane regions. There were no base pair variation in exons 1–4 and three base changes (G4014A, G4136A and C4152T) in exon 5 by sequencing and the three mutations may have some correlation with sexual precocity in goats. For the 4152 locus, the Jining Grey goat does with genotype TT and CT had 1.02 and 0.84 (P < 0.01) kids more than those with genotype CC, respectively. No significant difference (P > 0.05) was found in litter size between TT and CT genotypes in Jining Grey goat. For the other two loci, no significant difference (P > 0.05) was found in litter size between different genotypes in Jining Grey goats. The present study preliminarily indicated an association between allele T of the 4152 locus in GPR54 and high litter size in Jining Grey goats.

Keywords

Goat GPR54 Sexual precocity Litter size PCR-RFLP 

Notes

Acknowledgments

This work was supported by National Key Technology R&D Program of China (No. 2008BADB2B01), National Natural Science Foundation of China (30871773), National High Technology Research and Development Program of China (2006AA10Z139), National Key Basic Research and Development Program of China (2006CB102105), the earmarked fund for Modern Agro-industry Technology Research System of China (nycytx-39).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • G. L. Cao
    • 1
  • M. X. Chu
    • 1
  • L. Fang
    • 1
  • T. Feng
    • 1
  • R. Di
    • 1
  • N. Li
    • 2
  1. 1.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.State Key Laboratory of Agricultural BiotechnologyChina Agricultural UniversityBeijingPeople’s Republic of China

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