Molecular Biology Reports

, Volume 38, Issue 4, pp 2739–2750 | Cite as

Molecular analysis of the PGYRP (proline-, glycine- and tyrosine-rich protein) gene family in soybean

  • Yingmei Feng
  • Hui Peng
  • Shichu Liang


The genes coding for PGYRPs (proline-, glycine- and tyrosine-rich proteins) are widely distributed across eukaryotes and have been proposed to have critical role in plant development, especially in response to environmental stresses. In this study, total of 12 soybean PGYRPs (GmPGYRP1–12) were identified from the soybean genome database for the first time and full-length cDNA and DNA sequences of GmPGYRP7 was cloned. GmPGYRP1–12 genes encoded a set of small predicted proteins (<120 aa) with molecular mass of 7.20–13.29 kDa and isoelectric point of 4.06–6.57. All GmPGYRPs contained three exons and two introns with fixed occurring sites within genomic DNA sequences. In the putative GmPGYRP sequences, 4 amino acids (proline, glycine, tyrosine, and glutamine) account for more than 39% of the total protein composition. GmPGYRPs had a relatively flexible GYPPX motif followed by a highly conserved cysteine-rich domain (GCLAAXCCCCXLXC) and showed high similarity to other known PGYRPs, especially in C-terminal region. Most of PGYRPs can be divided into five subgroups according to phylogenetic analysis. The transcripts of GmPGYRP1, 3, 5, and 7, representing different PGYRP subgroups, appeared in different organs including seedling leaves, stems, roots, flowers, and developing seeds, but mainly accumulated in seedling roots. Furthermore, the expression of GmPGYRP1, 3, 5, and 7 was significantly regulated by drought, salt and cold, but obviously repressed by abscisic acid (ABA) at early stage. Our data suggest that GmPGYRP genes encoding a class of conservative XYPPX-repeat proteins probably play an important role in plant development as well as in response to abiotic stresses.


Abiotic stresses Proline-, glycine- and tyrosine-rich protein Soybean XYPPX motif 



Abscisic acid


Cysteine-rich transmembrane module


Days after flowering


Expressed sequence tag


Glycine- and proline-rich protein


Open reading frame


Polyethylene glycol


Proline-, glycine- and tyrosine-rich protein


Reverse transcriptase polymerase chain reaction


Untranslated region



This work was supported by the research funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, China, and the foundation of grand science and technology special of Guangxi Beibu Gulf (2010GXNSFE013003).

Supplementary material

11033_2010_419_MOESM1_ESM.doc (124 kb)
Supplementary material 1 (DOC 124 kb)
11033_2010_419_MOESM2_ESM.txt (11 kb)
Supplementary material 2 (TXT 10 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of EducationCollege of Life Sciences, Guangxi Normal UniversityGuilinChina

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