Molecular Biology Reports

, Volume 38, Issue 3, pp 2203–2209 | Cite as

Population data for 17 short tandem repeat loci on Y chromosome in northern Croatia

  • Branka Gršković
  • Gordan Mršić
  • Ozren Polašek
  • Andro Vrdoljak
  • Siniša Merkaš
  • Šimun Anđelinović


Human Y-short tandem repeats (STRs) are tandem repeat arrays of two to seven base pair units on non-recombining region (NRY) of the human Y chromosome. Studies on Y-STR are interesting in both population genetics and forensics. The aim of this study was to investigate the population genetic properties of 17 STR loci on Y chromosome in the northern Croatia region. We carried out a statistical analysis of the data from previously performed genetic analysis collected during routine forensic work by the Forensic Science Centre “Ivan Vučetić”. A total of 220 unrelated healthy men from northern Croatia were selected for the purpose of this study. Genomic DNA was extracted using Chelex procedure from FTA® cards. Y-chromosomal STRs were determined using the AmpFISTR Yfiler PCR amplification kit. The haplotype frequencies were determined by direct counting and analyzed using Arlequin 3.1 and analysis of molecular variance calculated with the Y chromosome haplotype reference database online analysis tool. A total of 210 haplotypes were identified, 200 of which were unique. Total haplotype diversity was 0.995. Locus diversity varied from 0.331 for DYS392 to 0.783 for DYS385 locus. Allele frequencies diversity was 0.662. Discrimination capacity was 95.7%. The use of European minimal haplotype set indicated the most resemblance of this population to the Croatian capital of Zagreb, with modest resemblance to Bosnia and Herzegovina, Serbia and Hungary. This article provides the first overview of the Y chromosome STR variability in northern Croatia, thus providing the referent point for any future forensic and genetic epidemiology efforts in this region.


Y chromosome Short tandem repeat Population genetics Haplotypes Northern Croatia 



This study was funded by the Ministry of Interior. The authors thank Sunčica Papić and Marija Valić for technical support.


  1. 1.
    Tilford C, Kuroda-Kawaguchi T, Skaletsky H, Rozen S, Brown L, Rosenberg M, McPherson J, Wylle K, Sekhon M, Kucaba T, Waterston R, Page D (2001) A physical map of the human Y chromosome. Nature 409(6822):943–945. doi: 10.1038/35057170 PubMedCrossRefGoogle Scholar
  2. 2.
    Quintana-Murci L, Krausz C, McElreavey K (2001) The human Y chromosome: function, evolution and disease. Forensic Sci Int 118(2–3):169–181PubMedCrossRefGoogle Scholar
  3. 3.
    Rappold GA (1993) The pseudoautosomal regions of the human sex. Hum Genet 92(4):315–324PubMedCrossRefGoogle Scholar
  4. 4.
    Butler JM (2005) Forensic DNA typing. Biology, technology and genetics of STR markers, 2nd edn. Elsevier Academic Press, Burlington, MAGoogle Scholar
  5. 5.
    Butler JM (2003) Recent developments in Y-short tandem repeat and Y-single nucleotide polymorphism analysis. Forensic Sci Rev 15:91–111Google Scholar
  6. 6.
    Willuweit S, Roewer L (2007) Y chromosome haplotype reference database (YHRD): update. Forensic Sci Int Genet 1(2):83–87. doi: 10.1016/j.fsigen.2007.01.017 PubMedCrossRefGoogle Scholar
  7. 7.
    Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513PubMedGoogle Scholar
  8. 8.
    Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  9. 9.
    Slatkin M (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139(1):457–462PubMedGoogle Scholar
  10. 10.
    Gusmão L, Butler JM, Carracedo A, Gill P, Kayser M, Mayr WR, Morling N, Prinz M, Roewer L, Tyler-Smith C, DNA Schneider PM Commission of the International Society of Forensic Genetics (2006) DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Sci Int 157(2–3):187–197. doi: 10.1016/j.forsciint.2005.04.002 PubMedCrossRefGoogle Scholar
  11. 11.
    Ljubković J, Stipisić A, Sutlović D, Definis-Gojanović M, Bucan K, Anđelinović Š (2008) Y-chromosomal short tandem repeat haplotypes in southern Croatian male population defined by 17 loci. Croat Med J 49(2):201–206. doi: 10.3325/cmj.2008.2.201 PubMedCrossRefGoogle Scholar
  12. 12.
    Haliti N, Čarapina M, Mašić M, Strinović D, Klarić IM, Kubat M (2009) Evaluation of population variation at 17 autosomal STR and 16 Y-STR haplotype loci in Croatians. Forensic Sci Int Genet 3(4):e137–e138. doi: 10.1016/j.fsigen.2008.11.004 PubMedCrossRefGoogle Scholar
  13. 13.
    Lovrečić L, Ristić S, Brajenović B, Kapović M, Peterlin B (2005) Human Y-specific STR haplotypes in the Western Croatian population sample. Forensic Sci Int 149(2–3):257–261. doi: 10.1016/j.forsciint.2004.06.026 PubMedGoogle Scholar
  14. 14.
    Barać L, Peričić M, Martinović Klarić I, Janićijević B, Parik J, Rootsi S, Rudan P (2003) Y chromosome STRs in Croatians. Forensic Sci Int 138(1–3):127–133. doi: 10.1016/j.forsciint.2003.09.004 PubMedGoogle Scholar
  15. 15.
    Ballard DJ, Phillips C, Thacker CR, Robson C, Revoir AP, Syndercombe Court D (2005) Y chromosome STR haplotypes in three UK populations. Forensic Sci Int 152(2–3):289–305. doi: 10.1016/j.forsciint.2004.10.006 PubMedCrossRefGoogle Scholar
  16. 16.
    Ballard DJ, Phillips C, Wright G, Thacker CR, Robson C, Revoir AP, Court DS (2005) A study of mutation rates and the characterization of intermediate, null and duplicated alleles for 13 Y chromosome STRs. Forensic Sci Int 155(1):65–70. doi: 10.1016/j.forsciint.2004.12.012 PubMedCrossRefGoogle Scholar
  17. 17.
    Beer Z, Csete K, Varga T (2004) Y-chromosome STR haplotype in Szekely population. Forensic Sci Int 139(2–3):155–158. doi: 10.1016/j.forsciint.2003.10.010 PubMedCrossRefGoogle Scholar
  18. 18.
    Wolańska Nowak P, Branicki W, Parys Proszek A, Kupiec T (2009) A population data for 17 Y-chromosome STR loci in South Poland population sample—some DYS458.2 variants uncovered and sequenced. Forensic Sci Int Genet 4(1):e43–e44PubMedCrossRefGoogle Scholar
  19. 19.
    Myres NM, Ritchie KH, Lin AA, Hughes RH, Woodward SR, Underhill PA (2009) Y-chromosome short tandem repeat intermediate variant alleles DYS392.2, DYS449.2 and DYS385.2 delineate new phylogenetic substructure in human Y-chromosome haplogroup tree. Croat Med J 50(3):239–249. doi: 10.3325/cmj.2009.50.239 PubMedCrossRefGoogle Scholar
  20. 20.
    Allor C, Einum DD, Scarpetta M (2005) Identification and characterization of variant alleles at CODIS STR loci. J Forensic Sci 50(5):1128–1133PubMedCrossRefGoogle Scholar
  21. 21.
    Füredi S, Woller J, Pádár Z, Angyal M (1999) Y-STR haplotyping in two Hungarian populations. Int J Legal Med 113(1):38–42. doi: 10.1007/s004140050276 PubMedCrossRefGoogle Scholar
  22. 22.
    Marjanovic D, Fornarino S, Montagna S, Primorac D, Hadziselimovic R, Vidovic S, Pojskic N, Battaglia V, Achilli A, Drobnic K, Andjelinovic S, Torroni A, Santachiara Benerecetti AS, Semino O (2005) The peopling of modern Bosnia-Herzegovina: Y-chromosome haplogroups in the three main ethnic groups. Ann Hum Genet 69(Pt 6):757–763PubMedCrossRefGoogle Scholar
  23. 23.
    Sterlinko H, Pajnic IZ, Balazic J, Komel R (2001) Human Y-specific STR haplotypes in a Slovenian population sample. Forensic Sci Int 120(3):226–228PubMedCrossRefGoogle Scholar
  24. 24.
    Veselinovic IS, Zgonjanin DM, Maletin MP, Stojkovic O, Djurendic-Brenesel M, Vukovic RM, Tasic MM (2008) Allele frequencies and population data for 17 Y-chromosome STR loci in a Serbian population sample from Vojvodina province. Forensic Sci Int 176(2–3):e23–e28. doi: 10.1016/j.forsciint.2007.04.003 PubMedCrossRefGoogle Scholar
  25. 25.
    Roewer L, Croucher PJP, Willuweit S, Lu TT, Kayser M, Lessig R, De Knijff P, Jobling MA, Tyler Smith C, Krawczak M (2005) Signature of recent historical events in the European Y-chromosomal STR haplotype distribution. Hum Genet 116(4):279–291. doi: 10.1007/s00439-004-1201-z PubMedCrossRefGoogle Scholar
  26. 26.
    Gršković B, Mršić G (2010) Y chromosome: from evolution to forensics—an overview. Acta Med Croatica 64:33–40PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Branka Gršković
    • 1
  • Gordan Mršić
    • 2
    • 3
  • Ozren Polašek
    • 4
  • Andro Vrdoljak
    • 2
    • 3
  • Siniša Merkaš
    • 1
  • Šimun Anđelinović
    • 3
    • 5
  1. 1.Department of Biology and Fibers, Divison of BiologyForensic Science Centre “Ivan Vučetić”, General Police Directorate, Ministry of InteriorZagrebCroatia
  2. 2.Forensic Science Centre “Ivan Vučetić”, General Police Directorate, Ministry of InteriorZagrebCroatia
  3. 3.University Center for Forensic Sciences, University of SplitSplitCroatia
  4. 4.Department of Medical Statistics, Epidemiology and Medical Informatics Andrija Stampar School of Public Health, Medical School, University of ZagrebZagrebCroatia
  5. 5.Department of Pathology and Forensic MedicineSplit University Hospital and School of MedicineSplitCroatia

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