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Molecular Biology Reports

, Volume 38, Issue 3, pp 2193–2201 | Cite as

Upregulation of interleukin-1β production by 1,25-Dihydroxyvitamin D3 in activated human macrophages

  • Bit-Na-Ra Lee
  • Tae-Hwan Kim
  • Jae-Bum Jun
  • Dae-Hyun Yoo
  • Jin-Hyun Woo
  • Sung Jae Choi
  • Young Ho Lee
  • Gwan Gyu Song
  • Yongjin Kim
  • Ji Young Lee
  • Jeongwon Sohn
  • Jong Dae Ji
Article

Abstract

1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) plays important roles in the immune system. In contrast to its well known function in the adaptive immune system, much less is known about the immunoregulatory effects of 1,25(OH)2D3 in the innate immune system, especially on activated human macrophages. Here we found that 1,25(OH)2D3 strongly stimulated the production of interleukin-1β (IL-1β) in PMA-differentiated U937 cells and human monocyte-derived macrophages treated with lipopolysaccharide (LPS) or PMA. In this study, Erk1/2 appeared to mediate 1,25(OH)2D3-induced expression of IL-1β. Parallel to the increased production of IL-1β, 1,25(OH)2D3 increased the expression and phosphorylation of the CCAAT enhancer-binding protein β (C/EBPβ), which is one of the key transcriptional regulatory factors for IL-1β transcription. These results suggest that 1,25(OH)2D3 may function as a proinflammatory molecule in inflammatory macrophages.

Keywords

1,25-Dihydroxyvitamin D3 IL-1β Macrophages Inflammation 

Notes

Acknowledgments

This work was supported by a Korea University Grant.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Bit-Na-Ra Lee
    • 1
  • Tae-Hwan Kim
    • 1
  • Jae-Bum Jun
    • 1
  • Dae-Hyun Yoo
    • 1
  • Jin-Hyun Woo
    • 2
  • Sung Jae Choi
    • 2
  • Young Ho Lee
    • 2
  • Gwan Gyu Song
    • 2
  • Yongjin Kim
    • 3
  • Ji Young Lee
    • 3
  • Jeongwon Sohn
    • 3
  • Jong Dae Ji
    • 2
  1. 1.The Hospital for Rheumatic Diseases, Hanyang UniversitySeoulKorea
  2. 2.Division of RheumatologyCollege of Medicine, Korea UniversitySeoulKorea
  3. 3.Department of BiochemistryCollege of Medicine, Korea UniversitySeoulKorea

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