Molecular Biology Reports

, Volume 38, Issue 3, pp 1457–1462 | Cite as

Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin

  • Yan Zhou
  • Leike Li
  • Longbo Hu
  • Tao Peng


Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1), a type-II Golgi transmembrane protein of unknown function, is up-regulated in many cancers. Its Golgi luminal domain is potentially the major functional domain. The goal of this study is to identify the proteins interacting with GOLPH2. Using secretory GOLPH2 (sGOLPH2, amino acid residues 55–401) as bait, secretory clusterin (sCLU) was identified as one interacting candidate by yeast two-hybrid screening, and the coiled-coil domain of GOLPH2 was found to be sufficient for interaction with sCLU. The interaction between GOLPH2 and sCLU was confirmed intracellularly and extracellularly. The intracellular co-localization of GOLPH2 and sCLU in Golgi was also shown. These results can help in understanding the biological and pathological significance of GOLPH2.


GOLPH2 GP73 Clusterin Protein interaction Coiled-coil domain 



This work was supported by the National Science Foundation (309701647), the National Basic Research Program (973) (2009CB522300, 2010CB530100), and the Science and Technology Program of Guangzhou, China (2007Z1-E0111).


  1. 1.
    Kristiansen G, Fritzsche FR, Wassermann K, Jager C, Tolls A, Lein M, Stephan C, Jung K, Pilarsky C, Dietel M, Moch H (2008) GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br J Cancer 99:939–948. doi: 10.1038/sj.bjc.6604614 CrossRefPubMedGoogle Scholar
  2. 2.
    Li H, Wetten S, Li L, St Jean PL, Upmanyu R, Surh L, Hosford D et al (2008) Candidate single-nucleotide polymorphisms from a genomewide association study of Alzheimer disease. Arch Neurol 65:45–53. doi: 10.1001/archneurol.2007.3 CrossRefPubMedGoogle Scholar
  3. 3.
    Kladney RD, Bulla GA, Guo L, Mason AL, Tollefson AE, Simon DJ, Koutoubi Z, Fimmel CJ (2000) GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53–65. doi: 10.1016/S0378-1119(00)00136-0 CrossRefPubMedGoogle Scholar
  4. 4.
    Fritzsche FR, Riener MO, Dietel M, Moch H, Jung K, Kristiansen G (2008) GOLPH2 expression in renal cell cancer. BMC Urol 8:15. doi: 10.1186/1471-2490-8-15 CrossRefPubMedGoogle Scholar
  5. 5.
    Hu JS, Wu DW, Liang S and Miao XY (2009) GP73, a resident Golgi glycoprotein, is sensibility and specificity for hepatocellular carcinoma of diagnosis in a hepatitis B-endemic Asian population. Med Oncol doi: 10.1007/s12032-009-9215-y
  6. 6.
    Bachert C, Fimmel C, Linstedt AD (2007) Endosomal trafficking and proprotein convertase cleavage of cis Golgi protein GP73 produces marker for hepatocellular carcinoma. Traffic 8:1415–1423. doi: 10.1111/j.1600-0854.2007.00621.x CrossRefPubMedGoogle Scholar
  7. 7.
    Wright LM, Yong S, Picken MM, Rockey D, Fimmel CJ (2009) Decreased survival and hepato-renal pathology in mice with C-terminally truncated GP73 (GOLPH2). Int J Clin Exp Pathol 2:34–47PubMedGoogle Scholar
  8. 8.
    Shannan B, Seifert M, Leskov K, Willis J, Boothman D, Tilgen W, Reichrath J (2006) Challenge and promise: roles for clusterin in pathogenesis, progression and therapy of cancer. Cell Death Differ 13:12–19. doi: 10.1038/sj.cdd.4401779 CrossRefPubMedGoogle Scholar
  9. 9.
    Jones SE, Jomary C (2002) Clusterin. Int J Biochem Cell Biol 34:427–431. doi: 10.1016/S1357-2725(01)00155-8 CrossRefPubMedGoogle Scholar
  10. 10.
    Behrens P, Jeske W, Wernert N, Wellmann A (2001) Downregulation of clusterin expression in testicular germ cell tumours. Pathobiology 69:19–23. doi: 10.1159/000048753 CrossRefPubMedGoogle Scholar
  11. 11.
    Gu Y, Chen W, Zhao Y, Chen L, Peng T (2009) Quantitative analysis of elevated serum Golgi protein-73 expression in patients with liver diseases. Ann Clin Biochem 46:38–43CrossRefPubMedGoogle Scholar
  12. 12.
    Kladney RD, Cui X, Bulla GA, Brunt EM, Fimmel CJ (2002) Expression of GP73, a resident Golgi membrane protein, in viral and nonviral liver disease. Hepatology 35:1431–1440. doi: 10.1053/jhep.2002.32525 CrossRefPubMedGoogle Scholar
  13. 13.
    Hogasen K, Homann C, Mollnes TE, Graudal N, Hogasen AK, Hasselqvist P, Thomsen AC, Garred P (1996) Serum clusterin and vitronectin in alcoholic cirrhosis. Liver 16:140–146. doi: 10.1111/j.1600-0676.1996.tb00719.x PubMedGoogle Scholar
  14. 14.
    Riener MO, Stenner F, Liewen H, Soll C, Breitenstein S, Pestalozzi BC, Samaras P, Probst-Hensch N, Hellerbrand C, Mullhaupt B, Clavien PA, Bahra M, Neuhaus P, Wild P, Fritzsche F, Moch H, Jochum W, Kristiansen G (2009) Golgi phosphoprotein 2 (GOLPH2) expression in liver tumors and its value as a serum marker in hepatocellular carcinomas. Hepatology 49:1602–1609. doi: 10.1002/hep.22843 CrossRefPubMedGoogle Scholar
  15. 15.
    Wright LM, Huster D, Lutsenko S, Wrba F, Ferenci P, Fimmel CJ (2009) Hepatocyte GP73 expression in Wilson disease. J Hepatol 51:557–564. doi: 10.1016/j.jhep.2009.05.029 CrossRefPubMedGoogle Scholar
  16. 16.
    Panico F, Rizzi F, Fabbri LM, Bettuzzi S, Luppi F (2009) Clusterin (CLU) and lung cancer. Adv Cancer Res 105:63–76. doi: 10.1016/S0065-230X(09)05004-0 CrossRefPubMedGoogle Scholar
  17. 17.
    Lau SH, Sham JS, Xie D, Tzang CH, Tang D, Ma N, Hu L, Wang Y, Wen JM, Xiao G, Zhang WM, Lau GK, Yang M, Guan XY (2006) Clusterin plays an important role in hepatocellular carcinoma metastasis. Oncogene 25:1242–1250. doi: 10.1038/sj.onc.1209141 CrossRefPubMedGoogle Scholar
  18. 18.
    Ghiggeri GM, Bruschi M, Candiano G, Rastaldi MP, Scolari F, Passerini P, Musante L, Pertica N, Caridi G, Ferrario F, Perfumo F, Ponticelli C (2002) Depletion of clusterin in renal diseases causing nephrotic syndrome. Kidney Int 62:2184–2194. doi: 10.1046/j.1523-1755.2002.00664.x CrossRefPubMedGoogle Scholar

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© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.State Key Laboratory of Respiratory Diseases, Laboratory of Viral ImmunologyGuangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of SciencesGuangzhouPeople’s Republic of China
  2. 2.University of Science and Technology of ChinaHefeiPeople’s Republic of China
  3. 3.Guangzhou Institute of Biomedicine and HealthGuangzhou Science ParkPeople’s Republic of China

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