Molecular Biology Reports

, Volume 38, Issue 2, pp 755–759 | Cite as

The polymorphisms of bovine VEGF gene and their associations with growth traits in Chinese cattle

  • Yonghong Pang
  • Juqiang Wang
  • Chunlei Zhang
  • Chuzhao Lei
  • Xianyong Lan
  • Wangping Yue
  • Chuanwen Gu
  • Danxia Chen
  • Hong ChenEmail author


PCR-SSCP and DNA sequencing methods were employed to screen the genetic variation of VEGF gene in 671 individuals belonging to three Chinese indigenous cattle breeds including Nanyang, Jiaxian Red and Qinchuan. Three haplotypes (A, B and C), four observed genotypes (AA, AB, BB and AC) and three new SNPs (6765T>C ss130456744, 6860A>G ss130456745, 6893T>C ss130456746) were detected. The analysis suggested that one SNP (ss130456744) in the bovine VEGF gene had significant effects on birth weight, body weight and heart girth at 6 months old in the Nanyang breed (P < 0.05). The results showed that the SNP (ss130456744) in intron 2 of the VEGF gene is associated with early development and growth of Chinese cattle. These findings raise hope that this polymorphism can be a molecular breeding marker in breeding strategies through marker assisted selection (MAS) in Chinese domestic cattle.


Vascular endothelial growth factor (VEGF) gene Single nucleotide polymorphism (SNP) Chinese cattle 



This study was supported by the National 863 Program of China (No. 2006AA10Z197, 2008AA101010), National Natural Science Foundation of China (No. 30771544, 30972080), National Key Technology R&D Program (No. 2006BAD01A10-5), Keystone Project of transfer gene in China (2009ZX08009-157B, 2008ZX08007-002), “13115” Sci-Tech Innovation Program of Shaanxi Province (2008ZDKG-11), Program of National Beef Cattle Industrial Technology System, Basic and Foreland Technology Study Program of Henan Province (No. 072300430160).

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yonghong Pang
    • 1
    • 2
  • Juqiang Wang
    • 3
  • Chunlei Zhang
    • 2
  • Chuzhao Lei
    • 1
  • Xianyong Lan
    • 1
  • Wangping Yue
    • 2
  • Chuanwen Gu
    • 2
  • Danxia Chen
    • 2
  • Hong Chen
    • 1
    Email author
  1. 1.Shaanxi Key Laboratory of Molecular Biology for AgricultureCollege of Animal Science and Technology, Northwest A&F UniversityYanglingChina
  2. 2.Institute of Cellular and Molecular BiologyXuzhou Normal UniversityXuzhouChina
  3. 3.Research Center of Cattle Engineering Technology in HenanZhengzhouChina

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