Molecular Biology Reports

, Volume 38, Issue 1, pp 621–629

Identification of genes differentially expressed in cauliflower associated with resistance to Xanthomonas campestris pv. campestris

  • Hanmin Jiang
  • Wenqin Song
  • Ai Li
  • Xiao Yang
  • Deling Sun
Article
  • 490 Downloads

Abstract

Black rot, caused by Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc), is one of the most damaging diseases of cauliflower and other crucifers. In order to investigate the molecular resistance mechanisms and to find the genes related to black rot resistance in cauliflower, a suppression subtractive hybridization (SSH) cDNA library was constructed using resistant line C712 and its susceptible near-isogenic line C731 as tester and driver, respectively. A total of 280 clones were obtained from the library by reverse northern blotting. Sequencing analysis and homology searching showed that these clones represent 202 unique sequences. The library included many defense/disease-resistant related genes, such as plant defensin gene PDF1.2, lipid transfer protein, thioredoxin h. Gene expression profiles of 12 genes corresponding to different functional categories were monitored by real-time RT-PCR. The results showed that the expression induction of these genes in the susceptible line C712 in response to Xcc was quicker and more intense, while in C731 the reaction was delayed and limited. Our results imply that these up-regulated genes might be involved in cauliflower responses against Xcc infection. Information obtained from this study could be used to understand the molecular mechanisms of disease response in cauliflower under Xcc stress.

Keywords

Black rot Brassica oleracea var. botrytis L. Suppression subtractive hybridization Xanthomonas campestris pv. campestris (Pammel) Dowson 

Abbreviations

CaR

Extracellular Ca2+-sensing receptors

CHI

Chalcone-flavanone isomerase

GSH-Px

Glutathione peroxidase

GST

Glutathione S-transferase

LTP

Lipid transfer protein

MIPS

Munich information center for protein sequences

NILs

Near isogenic lines

ROS

Reactive oxygen species

RubisCO

Ribulose-1,5-bisphosphate carboxylase/oxygenase

SSH

Suppression subtractive hybridization

TLP

Thaumatin-like protein

Trx

Thioredoxin

Xcc

Xanthomonas campestris pv. campestris (Pammel) Dowson

Supplementary material

11033_2010_148_MOESM1_ESM.doc (140 kb)
Supplementary material 1 (DOC 141 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hanmin Jiang
    • 1
  • Wenqin Song
    • 1
  • Ai Li
    • 1
  • Xiao Yang
    • 1
  • Deling Sun
    • 2
  1. 1.College of Life SciencesNankai UniversityTianjinPeople’s Republic of China
  2. 2.Tianjin Kernel Vegetable Research InstituteTianjinPeople’s Republic of China

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