Molecular Biology Reports

, Volume 37, Issue 8, pp 3983–3989 | Cite as

Interleukin-8-251A/T polymorphism and Helicobacter pylori infection influence risk for the development of gastric cardiac adenocarcinoma in a high-incidence area of China

  • Liwei Zhang
  • Chaoqun Du
  • Xiaoqing Guo
  • Li Yuan
  • Weiwei Niu
  • Weifang Yu
  • Limian Er
  • Shijie Wang


Polymorphisms in cytokine genes may contribute to increased susceptibility to different cancers. The aim of this paper is to investigate the association of IL-8-251A/T polymorphism and Helicobacter pylori (H. pylori) infection with the risk of developing gastric cardiac adenocarcinoma (GCA) in the south of Taihang Mountain, a high-incidence area of esophageal cancer in China. The IL-8-251 A/T polymorphism was genotyped in 519 cases of GCA and 504 healthy controls. The H. pylori infection in GCA patients and controls was detected by rapid urease test (RUT), histopathology or 14C-urea breath test (14C-UBT). The results showed that family history of upper gastrointestinal cancer (UGIC) and H. pylori infection significantly increased the risk of developing GCA. The overall genotype and allelotype distributions of IL-8 promoter SNPs in GCA patients were significantly different from those in healthy controls. Compared with TT genotype, AA genotype significantly elevated the risk of developing GCA. The stratification analysis revealed that, compared with the TT genotype, the AA genotype significantly elevated the risk of developing GCA in both positive family history of UGIC and H. pylori infection subgroups. This study provides evidence to support a relationship of increased susceptibility to GCA in individuals of the south Taihang Mountain region with IL-8 251 AA genotype, especially for those individuals who have family history of UGIC or H. pylori infection.


Gastric cardiac adenocarcinoma Interleukin-8 H. pylori Single nucleotide polymorphism 



The authors would like to thank Dr. Joshua C. Kwekel (Center for Functional Genomics, National Center for Toxicological Research, Food and Drug Administration, USA) for his helpful comments.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Liwei Zhang
    • 1
  • Chaoqun Du
    • 1
  • Xiaoqing Guo
    • 1
  • Li Yuan
    • 1
  • Weiwei Niu
    • 1
  • Weifang Yu
    • 1
  • Limian Er
    • 1
  • Shijie Wang
    • 1
  1. 1.Department of Endoscopy, The 4th Affiliated HospitalHebei Medical UniversityShijiazhuangPeople’s Republic of China

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