Molecular Biology Reports

, Volume 37, Issue 8, pp 3851–3862 | Cite as

Molecular analysis and expression of a floral organ-specific polygalacturonase gene isolated from rapeseed (Brassica napus L.)

  • Lili Wan
  • Xiuyun Xia
  • Dengfeng Hong
  • Guangsheng Yang
Article

Abstract

High throughput screening of stage-specific differentially expressed genes in a Brassica napus two-line Rs1046A/B subtractive library was used to identify the BnQRT3 gene associated with cell wall metabolism. Phylogenetic analysis indicates the protein product of BnQRT3 is polygalacturonase. According to cytological comparisons of Rs1046 sterile and fertile anthers, RT–PCR studies and in situ hybridizations, BnQRT3 is expressed most strongly in floral organs and may play an essential role in pollen maturation. Analysis of the histological staining pattern of BnQRT3 promoter-GUS constructs in transgenic Arabidopsis and Brassica napus revealed that proximal part of 5′-flanking region directed expression in the vascular tissue of filaments, veins in sepal and petals, stigma, branch connective and the floral organ abscission zone during the open flower stage. In the meanwhile, Activity of BnQRT3 was detected in the anthers, which commences at the microsporocyte stage and persists as anther approaches dehiscence. Strong GUS expression also can be observed in the vascular tissue of leaves and stem by compression with forceps or excision, suggesting that the BnQRT3 promoter is responsive to wounding.

Keywords

Floral organ BnQRT3 Promoter Vascular tissue Wounding 

Supplementary material

11033_2010_41_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 32 kb)
11033_2010_41_MOESM2_ESM.doc (711 kb)
Supplementary material 2 (DOC 711 kb)
11033_2010_41_MOESM3_ESM.doc (388 kb)
Supplementary material 3 (DOC 388 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Lili Wan
    • 1
  • Xiuyun Xia
    • 1
  • Dengfeng Hong
    • 1
  • Guangsheng Yang
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

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