Molecular Biology Reports

, 37:1143 | Cite as

A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina



It has been demonstrated that the duplicated carbonic anhydrase is induced by salt in the Dunaliella salina (D. salina) and duplicated carbonic anhydrase 1 (DCA1) is a member of carbonic anhydrase family. The purpose of this study was to identify whether both the DCA1 gene and its promoter from D. salina are salt-inducible. In this study, the results of real time RT–PCR showed that the transcripts of DCA1 were induced by gradient concentration of sodium chloride. Subsequently, a structurally novel promoter containing highly repeated GT/AC sequences of the DCA1 gene was isolated, which was able to drive a stable expression of the foreign bar gene in transformed cells of D. salina, and the gradient concentrations of sodium chloride in media paralleled regulations in the levels of both proteins and mRNA of the bar gene driven by the DCA1 promoter. Furthermore, analysis of GUS activities revealed that the salt-inducible expression of the external gus gene was regulated by the promoter fragments containing highly repeated GT sequences, but not by the promoter fragments deleting highly repeated GT sequences. The findings above-mentioned suggest that the highly repeated GT sequence in the DCA1 promoter is involved in the salt-inducible regulation in D. salina and may be a novel salt-inducible element.


bar gene Dunaliella salina Duplicated carbonic anhydrase 1 Promoter 



Duplicated carbonic anhydrase 1


Phosphinothricin acetyl transferase




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© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Laboratory for Cell Biology, Department of BiologyZhengzhou UniversityZhengzhouPeople’s Republic of China
  2. 2.Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiChina

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