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Molecular Biology Reports

, Volume 37, Issue 6, pp 2967–2973 | Cite as

CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA

  • Liping Yang
  • Ning Liu
  • Xiang Hu
  • Wenfeng Zhang
  • Tao Wang
  • Hong Li
  • Bo Zhang
  • Shuanglin Xiang
  • Jianlin Zhou
  • Jian ZhangEmail author
Article

Abstract

TNFAIP1 is a protein which can be induced by tumor necrosis factorα (TNFα) and interleukin-6 (IL-6), it may play roles in DNA synthesis, DNA repair, cell apoptosis and human diseases. However, very little has been known about how TNFAIP1 acts in these physiological processes. In this paper, CK2β was identified as a partner of TNFAIP1 by screening the HeLa cDNA library in yeast two-hybrid system with TNFAIP1 as a bait. Furthermore, it was demonstrated that CK2 could phosphorylate TNFAIP1 in vitro and in vivo, which facilitated the distribution of TNFAIP1 in nucleus and enhanced its interaction with PCNA. It is suggested that the phosphorylation of TNFAIP1 may be required for its functions.

Keywords

CK2 Phosphorylation TNFAIP1 Localization PCNA 

Notes

Acknowledgments

This work was supported by the 973 project of Ministry of Science and Technique of China (No. 2005CB522505, 2006CB943506, 2008CB517306), the National Natural Science Foundation of China (No. 90608006), the Hunan provincial doctorial innovative Foundation (2008XC065025), and the Hunan Provincial Natural Science Foundation of China (07JJ3075).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Liping Yang
    • 1
  • Ning Liu
    • 1
  • Xiang Hu
    • 1
  • Wenfeng Zhang
    • 1
  • Tao Wang
    • 1
  • Hong Li
    • 1
  • Bo Zhang
    • 1
  • Shuanglin Xiang
    • 1
  • Jianlin Zhou
    • 1
  • Jian Zhang
    • 1
    Email author
  1. 1.Key laboratory of Protein Chemistry and Developmental Biology, Ministry of Education of China, Department of Biochemistry and Molecular Biology, College of Life ScienceHunan Normal UniversityChangshaChina

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