CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA
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TNFAIP1 is a protein which can be induced by tumor necrosis factorα (TNFα) and interleukin-6 (IL-6), it may play roles in DNA synthesis, DNA repair, cell apoptosis and human diseases. However, very little has been known about how TNFAIP1 acts in these physiological processes. In this paper, CK2β was identified as a partner of TNFAIP1 by screening the HeLa cDNA library in yeast two-hybrid system with TNFAIP1 as a bait. Furthermore, it was demonstrated that CK2 could phosphorylate TNFAIP1 in vitro and in vivo, which facilitated the distribution of TNFAIP1 in nucleus and enhanced its interaction with PCNA. It is suggested that the phosphorylation of TNFAIP1 may be required for its functions.
KeywordsCK2 Phosphorylation TNFAIP1 Localization PCNA
This work was supported by the 973 project of Ministry of Science and Technique of China (No. 2005CB522505, 2006CB943506, 2008CB517306), the National Natural Science Foundation of China (No. 90608006), the Hunan provincial doctorial innovative Foundation (2008XC065025), and the Hunan Provincial Natural Science Foundation of China (07JJ3075).
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