Molecular Biology Reports

, Volume 37, Issue 6, pp 2809–2816

ΦC31 integrase interacts with TTRAP and inhibits NFκB activation

Article

Abstract

Phage ΦC31 integrase-mediated gene delivery is believed to be safer than using retroviral vectors since the protein confines its insertion of the target gene to a limited number of sites in mammalian genomes. To evaluate its safety in human cells, it is important to understand the interactions between this integrase and cellular proteins. Here we show that ΦC31 integrase interacts with TTRAP as presented by yeast two-hybrid and co-immunoprecipitation assays. Reducing the expression of endogenous TTRAP can increase the efficiency of ΦC31 integrase-mediated integration. A possible effect of interaction between ΦC31 integrase and TTRAP was highlighted by the fact that ΦC31 integrase inhibited the NFκB activation mediated by IL-1 in a dose-dependent manner. Because low dose of ΦC31 integrase can mediate considerable recombination events, we suggest that low dose of ΦC31 integrase be used when this integrase is applied in human cells.

Keywords

ΦC31 integrase TTRAP NFκB 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Bing-yin Wang
    • 1
  • Guan-lan Xu
    • 1
  • Cai-hong Zhou
    • 1
  • Ling Tian
    • 1
  • Jing-lun Xue
    • 1
  • Jin-zhong Chen
    • 1
  • William Jia
    • 1
    • 2
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan UniversityShanghaiChina
  2. 2.Department of SurgeryUniversity of British ColumbiaVancouverCanada

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