Molecular Biology Reports

, Volume 37, Issue 6, pp 2711–2717 | Cite as

Molecular characterization, chromosomal location, alternative splicing and polymorphism of porcine GFAT1 gene

  • K. Liu
  • G. Wang
  • S. H. Zhao
  • B. Liu
  • J. N. Huang
  • X. Bai
  • M. Yu
Article
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Accepted:

Abstract

Glutamine: fructose-6-phosphate amidotransferase (GFAT) is the rate-limiting enzyme of the hexosamine synthesis pathway, which plays important roles in insulin resistance and glucose toxicity. GFAT1 is one of the two isoenzymes of GFAT. In the present study, we cloned cDNA sequence of the porcine GFAT1 gene and identified a GFAT1 splice variant (designed GFAT1-L) that contains a 54 bp insertion within the coding region. Nested RT–PCR revealed that GFAT1 was ubiquitously expressed in all tested tissues, but GFAT1-L was only expressed in skeletal muscle and heart, not in liver, spleen, lung, kidney, small intestine, stomach and fat tissue, suggested that GFAT1-L was selectively expressed in striate muscle in pig. Using both the somatic cell hybrid panel and radiation hybrid panel, the GFAT1 gene was mapped to porcine chromosome 3q21-q27, in which several significant QTLs for carcass traits were found. Among the SNPs we found in porcine GFAT1 gene, only the g. 101A>G polymorphism which located in intron 8 was polymorphic in two pig populations we investigated in the study. Association analyses revealed that the g. 101A>G polymorphism has a significant effect on lean meat percentage (P < 0.05), corrected backfat thickness (P < 0.05) and backfat at the rump (P < 0.05).

Keywords

GFAT1 Porcine Mapping Alternate splicing Association analysis 
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Notes

Acknowledgments

We would like to thank Dr. Martine Yerle (INRA, France) for supplying the somatic cell hybrid and IMpRH panel DNAs. We thank Mr. Xiaoyong Du for technical help. This research was supported by the National Natural Science Foundation of China (30771537) and Program for New Century Excellent Talents in University (NCET70570669), and Key Project of National Basic Research and Developmental Plan (2006CB102105) of china.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • K. Liu
    • 1
    • 2
    • 3
  • G. Wang
    • 3
  • S. H. Zhao
    • 1
    • 2
  • B. Liu
    • 1
    • 2
  • J. N. Huang
    • 1
    • 2
  • X. Bai
    • 1
    • 2
  • M. Yu
    • 1
    • 2
  1. 1.Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of EducationHuazhong (Central China) Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Key Laboratory of Swine Genetics and Breeding of Ministry of AgricultureHuazhong (Central China) Agricultural UniversityWuhanPeople’s Republic of China
  3. 3.Comparative Medical LaboratoryGuangdong Medical Laboratory Animal CenterGuangzhouPeople’s Republic of China

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