Molecular Biology Reports

, Volume 37, Issue 5, pp 2415–2421 | Cite as

Characterization of a novel human CDK5 splicing variant that inhibits Wnt/β-catenin signaling

  • Qiang Li
  • Xianghua Liu
  • Mingjun Zhang
  • Guangming Ye
  • Qian Qiao
  • Yichen Ling
  • Yanhua Wu
  • Yuanyuan Zhang
  • Long Yu


The cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases, playing an essential role in regulating cell-cycle progression. In our present work, human CDK5 and a novel CDK5 splicing variant, named as CDK5-SV, were cloned from the cDNA library of human testis. CDK5-SV lacking the exon 7 of CDK5 encodes a protein of 260 amino acids. Through RT-PCR analysis in different human tissues, CDK5-SV was found to be expressed in testis, skeletal muscle, colon, bone marrow and ovary, while CDK5 was ubiquitously expressed. Immunofluorescence experiment in HeLa cells showed that the subcellular localizations of CDK5-SV and CDK5 were totally different. CDK5 mainly located in the cytoplasm, while CDK5-SV accumulated in nucleus. Reporter gene assay showed that when co-transfected with β-catenin, CDK5 and CDK5-SV could both strongly inhibit the Wnt/β-catenin signaling pathway. Consistently, CDK5-SV could also interact with β-catenin as CDK5 does. Taken together, our findings suggest that CDK5-SV might also be a negative regulator of Wnt/β-catenin signaling pathway.


CDK5-SV Immunofluorescence Kinase Splicing variant Wnt/β-catenin signaling pathway 



Cyclin-dependent kinase


Cyclin-dependent kinase 5- splicing variant




Wingless-type MMTV integration site family



This work was supported by the National 973 program of China (2004CB518605), the National 863 project of China (2006AA020501), the National Key Sci-Tech Special Project of China (2008ZX10002-020), the Project of the Shanghai Municipal Science and Technology Commission (03dz14086) and the National Natural Science foundation of China (30024001, 30771188).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Qiang Li
    • 1
  • Xianghua Liu
    • 1
  • Mingjun Zhang
    • 1
  • Guangming Ye
    • 1
  • Qian Qiao
    • 1
  • Yichen Ling
    • 1
  • Yanhua Wu
    • 1
  • Yuanyuan Zhang
    • 1
  • Long Yu
    • 1
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan UniversityShanghaiPeople’s Republic of China

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