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Molecular Biology Reports

, Volume 37, Issue 5, pp 2183–2188 | Cite as

Development and application of a loop-mediated isothermal amplification method on rapid detection Escherichia coli O157 strains from food samples

  • Xihong Zhao
  • Yanmei Li
  • Li Wang
  • Lijun You
  • Zhenbo XuEmail author
  • Lin Li
  • Xiaowei HeEmail author
  • Yao Liu
  • Jihua Wang
  • Liansheng Yang
Article

Abstract

We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Escherichia coli O157 strains. Six primers, including outer primers, inner primers and loop primers, were specially designed for recognizing eight distinct sequences on three targets, which were rfbE, stx1 and stx2. The detection limits were found to be 100, 100 and 10 fg DNA/tube for rfbE, stx1 and stx2, respectively. Application of LAMP assays were performed on 417 food-borne E. coli strains, the sensitivity of LAMP assays for the rfbE, stx1 and stx2 was 100, 95.3 and 96.3%, and the negative predictive value was 100, 96.7 and 97.1%, respectively; with a 100% specificity and positive predictive value for all three targets.

Keywords

Loop-mediated isothermal amplification (LAMP) Escherichia coli O157 Rapid detection 

Notes

Acknowledgments

This work was supported by Science Foundation of Ministry of Education of China(706046), National Natural Science Foundation of China (20436020) and State Scholarship Fund of China Scholarship Council (2008615044).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Xihong Zhao
    • 1
  • Yanmei Li
    • 2
  • Li Wang
    • 3
  • Lijun You
    • 1
  • Zhenbo Xu
    • 1
    • 4
    Email author
  • Lin Li
    • 1
  • Xiaowei He
    • 1
    Email author
  • Yao Liu
    • 5
  • Jihua Wang
    • 6
  • Liansheng Yang
    • 1
  1. 1.College of Light Industry and Food SciencesSouth China University of TechnologyGuangzhouChina
  2. 2.Guangzhou Medical CollegeGuangzhouChina
  3. 3.Food Safety Key Laboratory of Guangdong Province, College of Food ScienceSouth China Agricultural UniversityGuangzhouChina
  4. 4.Department of Microbial Pathogenesis, Dental SchoolUniversity of Maryland-BaltimoreBaltimoreUSA
  5. 5.Zhongshan Supervision Testing Institute of Quality & MetrologyZhongshanChina
  6. 6.Guangzhou Wondfo Biotechnology CompanyGuangzhouChina

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