Molecular Biology Reports

, Volume 37, Issue 1, pp 497–500 | Cite as

Associated analysis of single nucleotide polymorphisms of IGF2 gene’s exon 8 with growth traits in Wuzhishan pig

  • Guanyu Hou
  • Dongjin Wang
  • Song Guan
  • Hongpu Zeng
  • Xianzhou Huang
  • Yuehui Ma
Article

Abstract

A new SNP located in IGF-2 gene of Wuzhishan pig was detected while a C → T silent mutation at position 17 of exon 8 was detected by Single-Strand Conformational Polymorphism (SSCP). The value of polymorphism information content (PIC) indicated that this locus had intermediate polymorphism information content (0.25 < PIC < 0.5). The results of the fitness of Hardy-Weinberg equilibrium was in disequilibrium (P < 0.01). SAS analysis together with the multiple comparisons between polymorphisms and growth traits showed that: the differences of carcass weight (P = 0.024), withers height (P = 0.037) and chest girth (P = 0.025) in 10-month-old generation group were very remarkable.

Keywords

Wuzhishan pig IGF2 gene Single nucleotide polymorphisms Growth traits Correlation analysis 

References

  1. 1.
    Van Laere A-S, Nguyen M, Braunschweig M et al (2003) A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425(6960):832–836. doi:10.1038/nature02064 CrossRefPubMedGoogle Scholar
  2. 2.
    Sophie D, Kaouthar A, Danielle S et al (2001) Overexpression of human insulin-like growth factor binding protein-1 in the mouse leads to nephron deficit. Pediatr Res 49(5):660–666. doi:10.1203/00006450-200105000-00009 CrossRefGoogle Scholar
  3. 3.
    Jin-Tae J, Örjan C, Anna T et al (1999) A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. Nat Genet 21(2):157–158. doi:10.1038/5938 CrossRefGoogle Scholar
  4. 4.
    Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York, pp 63–4811Google Scholar
  5. 5.
    Carine N, Laurence M, Benoît B et al (1999) An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nat Genet 21(2):155–156. doi:10.1038/5935 CrossRefGoogle Scholar
  6. 6.
    Vykoukalová Z, Knoll A, Dvořák J et al (2006) New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs. J Anim Breed Genet 123(3):204–207. doi:10.1111/j.1439-0388.2006.00580.x CrossRefPubMedGoogle Scholar
  7. 7.
    Valérie A, Minh N, Anne-Sophie VL et al (2002) Comparative sequence analysis of the INS-IGF2–H19 gene cluster in pigs. Mamm Genome 13(7):388–398. doi:10.1007/s00335-001-3059-x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Guanyu Hou
    • 1
  • Dongjin Wang
    • 1
  • Song Guan
    • 1
  • Hongpu Zeng
    • 1
    • 2
  • Xianzhou Huang
    • 1
    • 2
  • Yuehui Ma
    • 2
  1. 1.Tropical Crops Genetic Resources Institute, CATASDanzhouChina
  2. 2.Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina

Personalised recommendations