Molecular Biology Reports

, Volume 37, Issue 2, pp 745–753

Screening of genes induced by salt stress from Alfalfa

  • Houcong Jin
  • Yan Sun
  • Qingchuan Yang
  • Yuehui Chao
  • Junmei Kang
  • Hong Jin
  • Yan Li
  • Gruber Margaret
Article

Abstract

An alfalfa cDNA library induced by salt stress was constructed by suppression subtraction hybridization (SSH) technology. Total RNA from 10-day-old seedlings was used as a “driver,” and total RNA from seedlings induced by salt was used as a “tester”. One hundred and nineteen clones identified as positive clones by reverse Northern dot-blotting resulted in 82 uni-ESTs comprised of 16 contigs and 66 singletons. Blast analysis of deduced protein sequences revealed that 51 ESTs had identity similar to proteins with known function, while 24 could not be annotated at all. Most of the annotated sequences were homologous to genes involved in abiotic or biotic stress in plants. Among these proteins, beta-amylase, fructose-1,6-bisphosphate, aldolase, and sucrose synthase are related to osmolyte synthesis; a CCCH-type zinc finger protein, DNA binding protein, His–Asp phosphotransfer protein, and the RelA/SpoT protein partake in transcription regulation and signal transduction; and ribulose-l,5-bisphosphate carboxylase/oxygenase, chlorophyll a/b binding proteins, and an early light-inducible proteins are related to photosynthesis. In addition, several ESTs, similar to genes from other plant species, closely involved in salt stress were isolated from alfalfa, such as an aquaporin protein, a late embryogenesis-abundant protein, and glutathione peroxidase.

Keywords

Suppression subtractive hybridization (SSH) Alfalfa (Medicago sativa L.) SMART technology Reverse Northern dot-blotting Salt stress Real-time PCR 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Houcong Jin
    • 1
    • 3
  • Yan Sun
    • 2
  • Qingchuan Yang
    • 1
  • Yuehui Chao
    • 1
  • Junmei Kang
    • 1
  • Hong Jin
    • 3
  • Yan Li
    • 1
  • Gruber Margaret
    • 4
  1. 1.Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.College of Animal Science and TechnologyChina Agriculture UniversityBeijingPeople’s Republic of China
  3. 3.College of AgronomyInner Mongolia Agricultural UniversityInner MongoliaPeople’s Republic of China
  4. 4.Saskatoon Research CentreAgriculture and Agri-FoodSaskatoonCanada

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