Methylenetetrahydrofolate reductase gene polymorphism in diabetes and obesity
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Methylenetetrahydrofolate reductase (MTHFR) polymorphism may play an important role in the pathophysiology of obesity and diabetes accompanied by obesity due to its influence on plasma homocysteine levels. There are significant and sometimes very strong relationship between levels of homocysteine and several multi-system diseases including CHD and CVA. To examine the association between MTHFR gene C677T polymorphism in diabetes and obesity with serum homocysteine levels. A total of 682 subjects were recruited in four groups (Normal, obese, diabetic and obese and diabetics). MTHFR gene C677T polymorphism was detected using PCR-RFLP technique. Serum homocysteine levels were measured using HPLC. There was a significant increase in the mean serum homocysteine levels in subjects carrying TT genotype (34.6 ± 26.5) compared to subjects carrying CC (15.1 ± 8) or CT genotype (16.4 ± 7.8) (P < 0.000). We found no significant differences for MTHFR allele and genotype frequencies between different groups. Our data have confirmed the association between serum homocysteine levels and MTHFR C677T genotype reported in other populations.
KeywordsMTHFR Polymorphism Homocysteine Diabetes Obesity
This study was funded by Endocrinology and Metabolism Research center of Tehran University of Medical Sciences. We thank the head, academic and support staff and laboratory of EMRC.
- 4.Freitas AI, Mendonca I, Guerra G, Brion M, Reis RP, Carracedo A, Brehm A (2008) Methylenetetrahydrofolate reductase gene, homocysteine and coronary artery disease: the A1298C polymprphism does matter. Inferences from a case study (Madeira, Portugal). Thromb Res 122:648–656. doi: 10.1016/j.thromres.2008.02.005 CrossRefPubMedGoogle Scholar
- 5.Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthew RG et al (1995) A candidate genetic risk factors for vascular disease: a common mutation in methylenetetrahydrofolate reductase: isolation of Cdna, mapping and mutation identification. Nat Genet 10:110–113. doi: 10.1038/ng0595-111 CrossRefGoogle Scholar
- 8.Inamoto N, Katsuya T, Kokubo Y, Mannami T, Asai T, Baba S et al (2003) Association of methylenetetrahydrofolate reductase gene polymorphism with carotid atherosclerosis depending on smoking status in a Japanese general population. Stroke 34:1628–1633. doi: 10.1161/01.STR.0000075769.09092.82 CrossRefPubMedGoogle Scholar
- 9.Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A, Allen RH (1993) Total homocysteine in plasma and serum: methods and clinical applications. Clin Biochem 93:1764–1779Google Scholar
- 10.Guttormsen AB, Ueland PM, Nesthus I, Nygard O, Schneede J, Vollset S et al (1996) Determinants and vitamin responsiveness of intermediate hyperhomocysteinemia (equal to or greater than 40 micromole/liter): the Hordaland homocysteine study. J Clin Invest 98:2174–2183. doi: 10.1172/JCI119024 CrossRefPubMedGoogle Scholar
- 13.Thawnashom K, Tungtrongchitr R, Petmitr S, Pongpaew P, Phonrat B, Tungtrongchitr A, Schelp FP (2005) Methylenetetrahydrofolate reductase (MTHFR) polymorphism (C677T) in relation to homocysteine concentration in overweight and obese Thais. Southeast Asian J Trop Med Public Health 36:459–466PubMedGoogle Scholar
- 21.Ruiz JR, Hurtig-Wennlof A, Ortega FB, Patterson E, Nilsson TK et al (2007) Homocysteine levels in children and adolescents are associated with the methylenetetrahydrofolate reductase 677C>T genotype, but not with physical activity, fitness or fatness: the European youth heart study. Br J Nutr 97:255–262. doi: 10.1017/S0007114507280535 CrossRefPubMedGoogle Scholar
- 23.Virtanen JK, Voutilainen S, Alfthan G, Korhonen MJ, Rissanen TH, Mursu J, Kaplan GA, Salonen JT (2005) Homocysteine as a risk factor for CVD mortality in men with other CVD risk factors: the Kuopio ischaemic heart disease risk factor (KIHD) study. J Intern Med 257:255–262. doi: 10.1111/j.1365-2796.2005.01450.x CrossRefPubMedGoogle Scholar
- 25.Ford ES, Smith SJ, Stroup DF, Steinberg KK, Mueller PW, Thacker SB (2002) Homocyst(e)ine and cardiovascular disease: a systematic review of the evidence with special emphasis on case–control studies and nested case–control studies. Int J Epidemiol 31:59–70. doi: 10.1093/ije/31.1.59 CrossRefPubMedGoogle Scholar