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Molecular Biology Reports

, Volume 37, Issue 1, pp 105–109 | Cite as

Methylenetetrahydrofolate reductase gene polymorphism in diabetes and obesity

  • Javad Tavakkoly Bazzaz
  • Mahnaz Shojapoor
  • Habibollah Nazem
  • Parvin Amiri
  • Hossein Fakhrzadeh
  • Ramin Heshmat
  • Maryam Parvizi
  • Shirin Hasani Ranjbar
  • Mahsa M. Amoli
Article

Abstract

Methylenetetrahydrofolate reductase (MTHFR) polymorphism may play an important role in the pathophysiology of obesity and diabetes accompanied by obesity due to its influence on plasma homocysteine levels. There are significant and sometimes very strong relationship between levels of homocysteine and several multi-system diseases including CHD and CVA. To examine the association between MTHFR gene C677T polymorphism in diabetes and obesity with serum homocysteine levels. A total of 682 subjects were recruited in four groups (Normal, obese, diabetic and obese and diabetics). MTHFR gene C677T polymorphism was detected using PCR-RFLP technique. Serum homocysteine levels were measured using HPLC. There was a significant increase in the mean serum homocysteine levels in subjects carrying TT genotype (34.6 ± 26.5) compared to subjects carrying CC (15.1 ± 8) or CT genotype (16.4 ± 7.8) (P < 0.000). We found no significant differences for MTHFR allele and genotype frequencies between different groups. Our data have confirmed the association between serum homocysteine levels and MTHFR C677T genotype reported in other populations.

Keywords

MTHFR Polymorphism Homocysteine Diabetes Obesity 

Notes

Acknowledgments

This study was funded by Endocrinology and Metabolism Research center of Tehran University of Medical Sciences. We thank the head, academic and support staff and laboratory of EMRC.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Javad Tavakkoly Bazzaz
    • 1
  • Mahnaz Shojapoor
    • 2
  • Habibollah Nazem
    • 2
  • Parvin Amiri
    • 1
  • Hossein Fakhrzadeh
    • 1
  • Ramin Heshmat
    • 1
  • Maryam Parvizi
    • 1
  • Shirin Hasani Ranjbar
    • 1
  • Mahsa M. Amoli
    • 1
    • 3
  1. 1.Endocrinology and Metabolism Research CentreTehran University of Medical SciencesTehranIran
  2. 2.Department of SciencePayamnoor UniversityTehranIran
  3. 3.EMRC, Dr Shariati HospitalTehranIran

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