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Molecular Biology Reports

, Volume 37, Issue 3, pp 1183–1190 | Cite as

ThPOD3, a truncated polypeptide from Tamarix hispida, conferred drought tolerance in Escherichia coli

  • Xiao-Hong Guo
  • Jing Jiang
  • Bai-Chen Wang
  • Hui-Yu Li
  • Yu-Cheng Wang
  • Chuan-Ping Yang
  • Gui-Feng LiuEmail author
Article

Abstract

The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS–PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were ~57, ~50 and ~47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.

Keywords

Peroxidase qRT-PCR Prokaryotic expression Abiotic stress Tamarix hispida 

Notes

Acknowledgments

This study was supported by national natural science foundation (Grant No. 30571509), Heilongjiang province scientific and technological project (Grant No. GB06B303 and WB07N02).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Xiao-Hong Guo
    • 1
  • Jing Jiang
    • 1
  • Bai-Chen Wang
    • 1
  • Hui-Yu Li
    • 1
  • Yu-Cheng Wang
    • 1
  • Chuan-Ping Yang
    • 1
  • Gui-Feng Liu
    • 1
    Email author
  1. 1.Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of EducationNortheast Forestry UniversityHarbinChina

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