A novel leucine-rich repeat receptor-like kinase gene in potato, StLRPK1, is involved in response to diverse stresses
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A potato gene, StLRPK1 (Solanum tuberosum L. leucine-rich-repeat receptor-like protein kinases 1), encoding a protein belonging to leucine-rich repeat receptor-like kinases (LRR-RLKs) was identified. It encodes 796 amino acids with 88% of identity to SRF3 of Arabidopsis thaliana and contains a signal peptide, five LRR motifs, a transmembrane domain, two proline-rich regions and a serine/threonine protein kinase domain. The transcripts were present at high levels in flowers and young leaves, while low in other tested organs. The mRNA of StLRPK1 was inducible in potato leaves by Phytophthora infestans, a pathogen causing late blight disease, and showed different profiles after treatment with salicylic acid, methyl jasmonate, ethylene, abscissic acid, wounding, 40°C, 4°C and a salinity stress. The results suggest that StLRPK1 may participate in the responses against environmental stresses and disease resistance in potato.
KeywordsStLRPK1 Leucine-rich-repeat receptor-like kinases RACE Biotic and abiotic stresses Solanum tuberosum L.
We thank the International Potato Center (CIP) for providing the potato clone 386209.10 with moderate late blight resistance. This work was funded by a grant from the National 863 Project of China (No. 2006AA10Z149). We also thank the anonymous reviewers for comments on the manuscript.
- 8.Kachroo A, Nasrallah ME, Nasrallah JB (2002) Self-incompatibility in the Brassicaceae: receptor-ligand signaling and cell-to-cell communication. Plant Cell 14(Supplement):227–238Google Scholar
- 11.Wang BL, Liu J, Tian ZD, Song BT, Xie CH (2005) Monitoring the expression patterns of potato genes associated with quantitative resistance to late blight during Phytophthora infestans infection using cDNA microarrays. Plant Sci 169:1155–1167. doi: 10.1016/j.plantsci.2005.07.020 CrossRefGoogle Scholar
- 13.Tian ZD, Liu J, Xie CH (2003) Isolation of resistance-related genes to P. infestans with suppression subtractive hybridization in the R-gene-free potato. Acta Genetica Sin 30:597–605 in Chinese with an English abstractGoogle Scholar
- 26.Battaglia M, Solórzano RM, Hernández M, Cuéllar-Ortiz S, García-Gómez B, Márquez J, Covarrubias AA (2007) Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings. Planta 225:1121–1133. doi: 10.1007/s00425-006-0423-9 CrossRefPubMedGoogle Scholar
- 29.Turner JG, Ellis C, Devoto A (2002) The jasmonate signal pathway. Plant Cell 14(Supplement):153–164Google Scholar