Molecular Biology Reports

, 36:2183 | Cite as

Selection of housekeeping genes for gene expression studies on the development of fruit bearing shoots in Chinese jujube (Ziziphus jujube Mill.)

  • Hai-feng Sun
  • Yu-ping Meng
  • Gui-mei Cui
  • Qiu-fen Cao
  • Jie Li
  • Ai-hua Liang


Chinese jujube (Ziziphus jujube Mill.) is important economically for its fruit and also bears attractive medical value. Its flower development concurs with the growth of current fruit bearing shoots (FBSs). However, events involved in current FBSs, including FBS differentiation, flower development, and reliable housekeeping gene are still unknown. In this study, the morphology of FBS development and floral formation were examined and expression profiles of several potential internal control genes was determined. These included genes encoding proteins involved in protein translation (translation elongation factor 1α, ZjEF1), protein folding (cyclophilin, ZjCyP) or degradation (ubiquitin extension protein, ZjUBQ), and proteins involved in the structure of the cytoskeleton (β-actin, ZjACT) or nucleosome (histone3, ZjH3). Our results showed that the floral development in early growing FBSs (less than 20 mm in length) or shoot apices was not complete. Among ZjACT, ZjEF1, ZjCyP, ZjUBQ, and ZjH3, ZjH3 was the most suitable housekeeping gene to evaluate FBS development, based on their expression in early growing FBSs, shoot apices, and different organs. These results will be useful for further molecular mechanism study about FBS development in Chinese jujube.


Chinese jujube Flower development Fruit bearing shoot Housekeeping gene Selection 



This work was supported by grants from the Shanxi Natural Science Foundation Committee of China (No. 20051081 and 20080011062-2).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Hai-feng Sun
    • 1
    • 2
    • 3
  • Yu-ping Meng
    • 2
  • Gui-mei Cui
    • 2
  • Qiu-fen Cao
    • 2
  • Jie Li
    • 4
  • Ai-hua Liang
    • 1
  1. 1.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi UniversityTaiyuanChina
  2. 2.Agri-Biotechnology Research Centre of Shanxi ProvinceTaiyuanChina
  3. 3.College of Chemistry and Chemical Engineering of Shanxi UniversityTaiyuanChina
  4. 4.Institute of Plant ProtectionShanxi Academy of Agricultural SciencesTaiyuanChina

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