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Molecular Biology Reports

, 36:2183 | Cite as

Selection of housekeeping genes for gene expression studies on the development of fruit bearing shoots in Chinese jujube (Ziziphus jujube Mill.)

  • Hai-feng Sun
  • Yu-ping Meng
  • Gui-mei Cui
  • Qiu-fen Cao
  • Jie Li
  • Ai-hua Liang
Article

Abstract

Chinese jujube (Ziziphus jujube Mill.) is important economically for its fruit and also bears attractive medical value. Its flower development concurs with the growth of current fruit bearing shoots (FBSs). However, events involved in current FBSs, including FBS differentiation, flower development, and reliable housekeeping gene are still unknown. In this study, the morphology of FBS development and floral formation were examined and expression profiles of several potential internal control genes was determined. These included genes encoding proteins involved in protein translation (translation elongation factor 1α, ZjEF1), protein folding (cyclophilin, ZjCyP) or degradation (ubiquitin extension protein, ZjUBQ), and proteins involved in the structure of the cytoskeleton (β-actin, ZjACT) or nucleosome (histone3, ZjH3). Our results showed that the floral development in early growing FBSs (less than 20 mm in length) or shoot apices was not complete. Among ZjACT, ZjEF1, ZjCyP, ZjUBQ, and ZjH3, ZjH3 was the most suitable housekeeping gene to evaluate FBS development, based on their expression in early growing FBSs, shoot apices, and different organs. These results will be useful for further molecular mechanism study about FBS development in Chinese jujube.

Keywords

Chinese jujube Flower development Fruit bearing shoot Housekeeping gene Selection 

Notes

Acknowledgments

This work was supported by grants from the Shanxi Natural Science Foundation Committee of China (No. 20051081 and 20080011062-2).

References

  1. 1.
    Altschul SF, Madden TL, Schaffer AA et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. doi: 10.1093/nar/25.17.3389 CrossRefPubMedGoogle Scholar
  2. 2.
    Brunner AM, Yakovlev IA, Strauss SH (2004) Validating internal controls for quantitative plant gene expression studies. BMC Plant Biol 4:14. doi: 10.1186/1471-2229-4-14 CrossRefPubMedGoogle Scholar
  3. 3.
    Burton RA, Shirley NJ, King BJ et al (2004) The CesA gene family of barley, quantitative analysis of transcripts reveals two groups of co-expressed genes. Plant Physiol 134:224–236. doi: 10.1104/pp.103.032904 CrossRefPubMedGoogle Scholar
  4. 4.
    Czechowski T, Stitt M, Altmann T et al (2005) Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139:5–17. doi: 10.1104/pp.105.063743 CrossRefPubMedGoogle Scholar
  5. 5.
    Dean JD, Goodwin PH, Hsiang T (2002) Comparison of relative RT-PCR and northern blot analyses to measure expression of b-1, 3-glucanase in Nicotiana benthamiana infected with Colletotrichum destructivum. Plant Mol Biol Rep 20:347–356. doi: 10.1007/BF02772122 CrossRefGoogle Scholar
  6. 6.
    Goncalves S, Cairney J, Maroco J et al (2005) Evaluation of control transcripts in real-time RT-PCR expression analysis during maritime pine embryogenesis. Planta 222:556–563. doi: 10.1007/s00425-005-1562-0 CrossRefPubMedGoogle Scholar
  7. 7.
    Gonzalez-Verdejo CI, Die JV, Nadal S et al (2008) Selection of housekeeping genes for normalization by real-time RT-PCR: analysis of Or-MYB1 gene expression in Orobanche ramose development. Anal Biochem 379(2):176–181. doi: 10.1016/j.ab.2008.05.003 CrossRefPubMedGoogle Scholar
  8. 8.
    Grbic V, Bleecker AB (2000) Axillary meristem development in Arabidopsis thaliana. Plant J 21:215–223. doi: 10.1046/j.1365-313x.2000.00670.x CrossRefPubMedGoogle Scholar
  9. 9.
    Jain M, Nijhawan A, Tyagi AK et al (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345:646–651. doi: 10.1016/j.bbrc.2006.04.140 CrossRefPubMedGoogle Scholar
  10. 10.
    Kim BR, Nam HY, Kim SU et al (2003) Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice. Biotechnol Lett 25:1869–1872. doi: 10.1023/A:1026298032009 CrossRefPubMedGoogle Scholar
  11. 11.
    Kotoda N, Wada M, Komori S et al (2000) Expression pattern of homologues of floral meristem identity genes LFY and AP1 during flower development in apple. J Am Soc Hortic Sci 125:398–403Google Scholar
  12. 12.
    Kotoda N, Wada M, Kusaba S et al (2002) Overexpression of MdMADS5, an APETALA1-like gene of apple, causes early flowering in transgenic Arabidopsis. Plant Sci 162:679–687. doi: 10.1016/S0168-9452(02)00024-9 CrossRefGoogle Scholar
  13. 13.
    Mimida N, Kidou S, Kotoda N (2007) Constitutive expression of two apple (Malus × domestica Borkh.) homolog genes of LIKE HETEROCHROMATIN PROTEIN1 affects flowering time and whole-plant growth in transgenic Arabidopsis. Mol Genet Genomics 278:295–305. doi: 10.1007/s00438-007-0250-0 CrossRefPubMedGoogle Scholar
  14. 14.
    Nicot N, Hausman J-F, Hoffmann L et al (2005) Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 56:2907–2914. doi: 10.1093/jxb/eri285 CrossRefPubMedGoogle Scholar
  15. 15.
    Qu ZZ, Wang YH, Zhou JZ (1963) A preliminary report on the observation of flower bud differentiation of Chinese jujube (in chinese). J Hebei Agr Univ 2:7–15Google Scholar
  16. 16.
    Qu ZZ, Wang YH, Zhou JZ (1981) Observasion of flower bud differentiation of Chinese jujube (second report) (in chinese). J Hebei Agr Univ 4:86–102Google Scholar
  17. 17.
    Reid KE, Olsson N, Schlosser J et al (2006) An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biol 6:27. doi: 10.1186/1471-2229-6-27 CrossRefPubMedGoogle Scholar
  18. 18.
    Schmittgen TD, Zakrajsek BA (2000) Effect on experimental treatment on housekeeping gene expression: validation by realtime, quantitative RT-PCR. J Biochem Biophys Methods 46:69–81. doi: 10.1016/S0165-022X(00)00129-9 CrossRefPubMedGoogle Scholar
  19. 19.
    Sturzenbaum SR, Kille P (2001) Control genes in quantitative molecular biological techniques: the variability of invariance. Comp Biochem Physiol B 130:281–289. doi: 10.1016/S1096-4959(01)00440-7 CrossRefPubMedGoogle Scholar
  20. 20.
    Thellin O, Zorzi W, Lakaye B et al (1999) Housekeeping genes as internal standards: use and limits. J Biotechnol 75:291–295. doi: 10.1016/S0168-1656(99)00163-7 CrossRefPubMedGoogle Scholar
  21. 21.
    Thomas C, Meyer D, Wolff M et al (2003) Molecular characterization and spatial expression of the sunflower ABP1 gene. Plant Mol Biol 52:1025–1036. doi: 10.1023/A:1025482432486 CrossRefPubMedGoogle Scholar
  22. 22.
    Volkov RA, Panchuk II, Schoffl F (2003) Heat-stress-dependency and developmental modulation of gene expression: the potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCR. J Exp Bot 54:2343–2349. doi: 10.1093/jxb/erg244 CrossRefPubMedGoogle Scholar
  23. 23.
    Warrington JA, Nair A, Mahadevappa M et al (2000) Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes. Physiol Genomics 2:143–147PubMedGoogle Scholar
  24. 24.
    Zhu C (1963) The application of PAS reaction as a stain technique in plant histology (in chinese). Acta Bot Sin 11:155–166Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Hai-feng Sun
    • 1
    • 2
    • 3
  • Yu-ping Meng
    • 2
  • Gui-mei Cui
    • 2
  • Qiu-fen Cao
    • 2
  • Jie Li
    • 4
  • Ai-hua Liang
    • 1
  1. 1.Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi UniversityTaiyuanChina
  2. 2.Agri-Biotechnology Research Centre of Shanxi ProvinceTaiyuanChina
  3. 3.College of Chemistry and Chemical Engineering of Shanxi UniversityTaiyuanChina
  4. 4.Institute of Plant ProtectionShanxi Academy of Agricultural SciencesTaiyuanChina

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