Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby
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Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.
KeywordsChalcone synthase gene Citrus sinensis (L.) Osbeck cv. Ruby Anthocyanins biosynthesis Fluorescence in situ hybridization (FISH)
The work was partially carried out in the Key Laboratory of MOE for Plant Development Biology in Wuhan University of China.
- 13.Ji Y, Jinxia H, Hongya G, Yang Z, Ziheng Y (2002) Duplication and adaptive evolution of the chalcone synthase genes of Dendranthema (Asteraceae). Mol Biol Evol 19:1752–1759Google Scholar
- 14.Fliegmann J, Schroder G, Schanz S, Britsch L, Schroder J (1992) Molecular analysis of chalcone and dihydropinosylvin synthase from Scots pine (Pinus sylvestris), and differential regulation of these and related enzyme activities in stressed plants. Plant Mol Biol 18:489–503. doi: 10.1007/BF00040665 PubMedCrossRefGoogle Scholar