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Molecular Biology Reports

, Volume 36, Issue 7, pp 1951–1956 | Cite as

Molecular characterization of Jatropha genetic resources through inter-simple sequence repeat (ISSR) markers

  • R. Senthil KumarEmail author
  • K. T. Parthiban
  • M. Govinda Rao
Article

Abstract

The genetic diversity among eight Jatropha species and three Jatropha curcas accessions were analyzed using ISSR-PCR. Nine ISSR primers generated reproducible amplification banding pattern of 61 polymorphic bands out of 64 scored accounting for 98.14% polymorphism across the species. The ISSR primers viz., I1, I2, I3, I4, I5, I6, I7 and I10 generated 100% polymorphic patterns. Jaccard’s coefficient of similarity varied from 0.346 to 0.807, indicative of high level of genetic variation among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters, one comprising all accessions of J. curcas L (TNMJ1, TNMJ 22 and TNMJ 23), while second included four species viz., J. tanjorensis J. L. Ellis et Saroja., J. gossypifolia L., J. podagrica Hook and J. maheshwarii Subrum and M.P. Nayer and the third cluster included another four species viz., J. villosa Wight J. multifida L., J. integerrima Jacq and J. glandulifera Roxb. The overall grouping pattern of clustering corresponds well with principal component analysis (PCA) confirming patterns of genetic diversity observed among the species. So far, there are no reports on the molecular diversity of the Jatropha species through ISSR marker. This study provides valid guidelines for collection, conservation and characterization of Jatropha genetic resources and also for further breeding programme towards biodiesel production.

Keywords

ISSR-PCR Jatropha species Genetic diversity Biodiesel 

Notes

Acknowledgments

The authors profusely thank DBT (Department of Biotechnology, India), New Delhi, for funding the project on “Breeding and Biotechnological Approaches for the Improvement of Jatropha Genetic resources”. The help rendered by Dr. S. Ganesh Ram, Dr. P. Hemalatha, Dr. S. Hariharan, Dr. Chezhiyan and Sundaramoorthi, TNAU, Coimbatore, India for their technical support is duly acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • R. Senthil Kumar
    • 1
    Email author
  • K. T. Parthiban
    • 1
  • M. Govinda Rao
    • 1
  1. 1.Forest College and Research InstituteMettupalayamIndia

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