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Molecular Biology Reports

, Volume 36, Issue 7, pp 1819–1824 | Cite as

Molecular characterization, chromosomal localization and association analysis with back-fat thickness of porcine LPIN2 and LPIN3

  • Xiaoping He
  • Xuewen Xu
  • Bang LiuEmail author
Article

Abstract

The products of mammalian LPIN2 and LPIN3 are phosphatidate phosphatase type 1 enzymes, which play an important role in the de novo biosynthesis of triacylglycerol, phosphatidylcholine and phosphatidylethanolamine. In this study, we obtained a 2,985-bp cDNA sequence of porcine LPIN2, which contains a 2,676-bp open reading frame flanked by an 11-bp 5′UTR and a 298-bp 3′UTR, and a 2,843-bp cDNA sequence of porcine LPIN3, which contains a 111-bp 5′UTR, a 2,580-bp open reading frame and a 152-bp 3′UTR. RT-PCR analysis showed that both LPIN2 and LPIN3 mRNA were ubiquitously expressed with a very high level in liver. By using the somatic cell hybrid panel (SCHP) and the radiation hybrid (IMpRH) panel, porcine LPIN2 and LPIN3 were assigned to 6q24-(1/2)q31 and 17(1/2)q21-q23, respectively. One T2193C single nucleotide polymorphism in LPIN2 was identified and was detected by Hin6I PCR-RFLP. Association analysis showed that different genotypes of LPIN2 were associated with back-fat thickness between the 6th and 7th ribs (P < 0.01).

Keywords

Pig LPIN2 LPIN3 Chromosomal localization SNP Association analysis 

Notes

Acknowledgments

The authors would like to thank Dr. Martine Yerle for providing the SCHP and RH panel (INRA, Castanet-Tolosan, France). The work was supported by the National Natural Science Foundation of China (30571007, 30771536) and the National High Science and Technology Foundation of China (2007AA10Z168).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Lab of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of EducationHuazhong Agricultural UniversityWuhanPR China

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