Molecular Biology Reports

, 36:1741 | Cite as

Response of cementoblast-like cells to mechanical tensile or compressive stress at physiological levels in vitro

  • Lan Huang
  • Yao Meng
  • Aishu Ren
  • Xianglong Han
  • Ding Bai
  • Lina Bao


To clarify the role of cementoblast in orthodontic-related root resorption, this study was attempted to examine whether murine cementoblast-like cells are responsive to mechanical stress, and how mechanical forces regulate bone sialoprotein (BSP) and osteopontin (OPN) gene expression in these cells in vitro. In this force-loading model, defined and reproducible mechanical loadings of different magnitudes and types were applied up to 24 h. Besides a transitory and reversible change in cell proliferation, remarkable alterations in gene transcription of BSP and OPN were found. BSP mRNA was suppressed by the stresses. Three and six hours-loadings at 2,000 μstrain up-regulated the expression of OPN mRNA, while the other loadings inhibited it. The study also concluded that 4,000 μstrain was likely to exert more influence on cementoblast-like cells than 2,000 μstrain. Furthermore, no obvious evidence indicated the difference between tension and compression. These results suggested that cementoblast-like cells are sensitive to mechanical stress, and may play a role in regulating orthodontic-related root resorption/repair.


Cementoblast Mechanical stress Proliferation mRNA Bone sialoprotein Osteopontin 


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Lan Huang
    • 1
    • 2
  • Yao Meng
    • 1
    • 2
  • Aishu Ren
    • 3
  • Xianglong Han
    • 1
    • 2
  • Ding Bai
    • 1
    • 2
  • Lina Bao
    • 4
  1. 1.State Key Laboratory of Oral DiseaseSichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of Orthodontics, West China Stomatology HospitalSichuan UniversityChengduPeople’s Republic of China
  3. 3.Department of Orthodontics, Chongqing Stomatology HospitalChongqing Medical UniversityChongqingPeople’s Republic of China
  4. 4.Department of OrthodonticsGuangdong Provincial Stomatological HospitalGuangzhouPeople’s Republic of China

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