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Molecular characterization of Echinococcus granulosus of Indian animal isolates on the basis of nuclear and mitochondrial genotype

  • J. Gudewar
  • D. Pan
  • A. K. Bera
  • S. K. Das
  • A. Konar
  • J. R. Rao
  • A. K. Tiwari
  • D. BhattacharyaEmail author
Article

Abstract

Sixty-six isolates of larval stage of Echinococcus granulosus, a known pathogenic parasite of man and animals were collected from cattle, buffalo, sheep, and goats. Single-stranded conformation polymorphism (SSCP) for analysis of variation after denaturation of amplicon of intron of actin II (ACTII) revealed six SSCP phenotypes. Intron portion was analyzed considering introns-early and introns-late theories. Isolates belonging to different conformers were further screened for mitochondrial ATPase subunit 6 (ATP6) and NADH dehydrogenase subunit II (nadII) genotypes. Assignment of each isolate to its specific strain was achieved after comparing with standard genotypes of E. granulosus. Variants deduced by nuclear targets did not match with mitochondrial haplotypes. A possible explanation for this observation can be attributed toward interspecific hybridization since cross-fertilization occurs less frequently in hermaphrodite organisms. A phylogenetic tree drawn on the basis of predicted aminoacid sequence of ATP6 and nadII revealed two distinct clusters i.e. E. granulosus sensu stricto and E. ortleppi/cattle strain (EG5). To the best of our knowledge, this is the first report of genetic characterization of two distinct ATP6 and nadII genotypes of zoonotic importance living in sympatry.

Keywords

Echinococcus granulosus Cattle Buffalo Sheep Goat India 

Notes

Acknowledgements

This study is supported by the Department of Biotechnology, Ministry of Science and Technology, Government of India. We also acknowledge the Director, Indian Veterinary Research Institute for providing infrastructural facilities. The first author would also like to acknowledge the Indian Council of Agricultural Research for the Junior Fellowship award granted in course of the Masters Degree Program.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • J. Gudewar
    • 1
  • D. Pan
    • 1
  • A. K. Bera
    • 1
  • S. K. Das
    • 1
  • A. Konar
    • 1
    • 2
  • J. R. Rao
    • 1
    • 3
  • A. K. Tiwari
    • 1
    • 3
  • D. Bhattacharya
    • 1
    Email author
  1. 1.Indian Veterinary Research InstituteKolkataIndia
  2. 2.Indian Institute of Chemical BiologyKolkataIndia
  3. 3.Indian Veterinary Research InstituteIzatnagarIndia

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