Serum levels of testosterone and gonadotrophins with respect to smoking status and genetic polymorphism of GSTT1

  • Mostafa SaadatEmail author


Objectives It is reported that parental exposure to toxicants can influence offspring sex ratio at birth. Studies have reported that several chemicals found in cigarette smoke are substrates of glutathione S-transferase T1 (GSTT1, a member of GSTθ). To determine the effect of cigarette smoke on serum levels of testosterone and gonadotrophins of smokers and possible association of these hormones levels with GSTT1 polymorphism, the present study was done. Methods Our study was conducted on 181 (40 smokers, 141 non-smokers) male subjects. Genomic DNA was extracted from peripheral blood. The GSTT1 genotyping was performed using PCR-based method. All measurements for testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) were done in one laboratory. Results In smoker subjects the mean ± sd of serum testosterone, FSH, and LH were 4.64 ± 1.63 ng/ml, 2.72 ± 1.17 IU/l, and 3.03 ± 1.04 IU/l, respectively. In non-smoker subjects the mean ± sd of serum testosterone, FSH, and LH were 4.49 ± 1.24 ng/ml, 2.89 ± 1.26 IU/l, and 3.07 ± 1.28 IU/l, respectively. There was no significant difference between smokers and non-smokers for serum testosterone (t = 0.622, df = 179, P = 0.535), FSH (t = −0.757, df = 179, P = 0.450), and LH (t = −0.179, df = 179, P = 0.858). Also there was no significant difference between smokers and non-smokers in either GSTT1 null or positive genotypes for levels of testosterone, FSH, and LH. Conclusion Based on present data, it might be concluded that serum levels of testosterone and gonadotrophins were not significantly different between smoker and non-smoker males in both null and present GSTT1 genotypes.


FSH GSTT1 LH Polymorphism Smoking Testosterone 



The author is indebted to the participants for their close cooperation. The author is indebted to Dr. Hassan Mohabtkar for critical reading of the manuscript and Dr. Maryam Ansari-Lari for her contribution in statistical analysis and discussion. I would like to acknowledge Mrs. Nasrin Monzavi for her assistance during the course of this study. This study was supported by Shiraz University.


  1. 1.
    James WH (2006) Offspring sex ratio at birth as markers of paternal endocrine disruption. Environ Res 100:77–85. doi: 10.1016/j.envres.2005.03.001 PubMedCrossRefGoogle Scholar
  2. 2.
    Fukuda M, Fukuda K, Shimizu T et al (2002) Parental periconceptional smoking and male: female ratio of newborn infants. Lancet 359:1407–1408. doi: 10.1016/S0140-6736(02)08362-9 PubMedCrossRefGoogle Scholar
  3. 3.
    Mills JL, England L, Granath F et al (2003) Cigarette smoking and the male-female sex ratio. Fertil Steril 79:1243–1245. doi: 10.1016/S0015-0282(03)00156-0 PubMedCrossRefGoogle Scholar
  4. 4.
    Parazzini F, Chatenoud L, Maffioletti C et al (2005) Periconceptional smoking and male:female ratio of newborns. Eur J Public Health 65:613–614. doi: 10.1093/eurpub/cki052 CrossRefGoogle Scholar
  5. 5.
    Hayes JD, Flanagan JU, Jowsey IR (2005) Glutathione transferases. Annu Rev Pharmacol Toxicol 45:51–88. doi: 10.1146/annurev.pharmtox.45.120403.095857 PubMedCrossRefGoogle Scholar
  6. 6.
    Ishigaki S, Abramortiz M, Listowsky I (1989) Glutathione S-transferases are major cytosolic thyroid hormone binding proteins. Arch Biochem Biophys 273:265–272. doi: 10.1016/0003-9861(89)90483-9 PubMedCrossRefGoogle Scholar
  7. 7.
    Listowsky I, Abramovitz M, Homma H et al (1988) Intracellular binding and transport of hormones and xenobiotics by glutathione S-transferases. Drug Metab Rev 19:305–318. doi: 10.3109/03602538808994138 PubMedCrossRefGoogle Scholar
  8. 8.
    Pemble S, Schroeder KR, Spencer SR et al (1994) Human glutathione S-transferase theta (GSTT1): cDNA cloning and the characterization of a genetic polymorphism. Biochem J 300:271–276PubMedGoogle Scholar
  9. 9.
    Ansari-Lari M, Saadat M, Hadi N (2004) Influence of GSTT1 null genotype on the offspring sex ratio of gasoline filling station workers. J Epidemiol Community Health 58:393–394. doi: 10.1136/jech.2003.011643 PubMedCrossRefGoogle Scholar
  10. 10.
    Saadat M, Ansari-Lari M, Bahaoddini A (2002) Sex ratio at birth in Masjid-i-Sulaiman (Khozestan province, Iran). Occup Environ Med 59:853. doi: 10.1136/oem.59.12.853 PubMedCrossRefGoogle Scholar
  11. 11.
    Saadat M, Bahaoddini A, Ansari-Lari M (2003) Possible effect of maternal hormones and GSTs genotypes on sex of offspring. Occup Environ Med 60:704. doi: 10.1136/oem.60.2.146-a CrossRefGoogle Scholar
  12. 12.
    Saadat M (2006) Genetic polymorphisms of glutathione S-transferase T1 (GSTT1) and susceptibility to gastric cancer, a meta-analysis. Cancer Sci 97:505–509. doi: 10.1111/j.1349-7006.2006.00207.x PubMedCrossRefGoogle Scholar
  13. 13.
    Saadat I, Saadat M (2001) Glutathione S-transferase M1 and T1 null genotypes and the risk of gastric and colorectal cancers. Cancer Lett 169:21–26. doi: 10.1016/S0304-3835(01)00550-X PubMedCrossRefGoogle Scholar
  14. 14.
    Abbas A, Delvinquoere K, Lechevrel M et al (2004) GSTM1, GSTT1, GSTP1, and CYP1A1 genetic polymorphisms and susceptibility to esophageal cancer in a French population: different pattern of squamous cell carcinoma and adenocarcinoma. World J Gastroenterol 10:3389–3393PubMedGoogle Scholar
  15. 15.
    Saadat M, Saadat I, Saboori Z et al (2004) Combination of CC16, GSTM1, and GSTT1 genetic polymorphisms is associated with asthma. J Allergy Clin Immunol 113:996–998. doi: 10.1016/j.jaci.2004.02.007 PubMedCrossRefGoogle Scholar
  16. 16.
    Saadat M, Ansari-Lari M (2007) Genetic polymorphisms of glutathione S-transfearse T1 (GSTT1), M1 (GSTM1) and asthma, a meta-analysis of the literature. Pak J Biol Sci 10:4183–4189PubMedCrossRefGoogle Scholar
  17. 17.
    Saadat M, Mobayen F, Frasshbandi H (2004) Genetic polymorphism of glutathione S-transferase T1: a candidate genetic modifier of individual susceptibility to schizophrenia. Psychiatry Res 153:87–91. doi: 10.1016/j.psychres.2006.03.024 CrossRefGoogle Scholar
  18. 18.
    Saadat M, Mohabatkar H (2004) Polymorphisms of glutathione S-transferases M1 and T1 do not account for interindividual differences for smoking behavior. Pharmacol Biochem Behav 77:793–795. doi: 10.1016/j.pbb.2004.02.003 PubMedCrossRefGoogle Scholar
  19. 19.
    Saadat M, Saadat I (2003) Offspring sex ratio is not associated with parental GSTT1 and GSTM1 null genotypes. Reprod Toxicol 17:345–347. doi: 10.1016/S0890-6238(03)00006-6 PubMedCrossRefGoogle Scholar
  20. 20.
    Saadat M, Monzavi N (2007) Genetic polymorphisms of glutathione S-transferase T1 (GSTT1) and alterations of sex hormones in filling station workers. Fertil Steril. doi: 10.1016/j.fertnstert.2007.06.024

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Biology, College of SciencesShiraz UniversityShirazIran
  2. 2.Institute of BiotechnologyShiraz UniversityShirazIran

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